Anti-acetyl Cortactin Antibody

32160702

NA.41

Cortactin is a cytoskeleton protein that facilitates assembly of cortical actin. Cortactin is widely expressed in most adherent cells and is a prominent substrate of protein-tyrosine kinase Src in vivo and in vitro. The protein sequence of cortactin contains multiple amino-terminal tandem repeats of a unique 37-amino acid sequence, which associates with actin, and a Src homology 3 domain at the carboxyl terminus.

Recent research efforts show cortactin is acetylated in vivo and is deacetylated by HDAC6 (Histone deacetylase 6). HDAC6 is a tubulin-specific deacetylase that regulates microtubule-dependent cell movement. In recent studies it was demonstrated that HDAC6 binds cortactin and alters the ability of cortactin to bind F-actin by modulating a “charge patch” in its repeat region. Findings suggest that, in addition to its role in microtubule-dependent cell motility, HDAC6 influences actin-dependent cell motility by altering the acetylation status of cortactin, which, in turn, changes the F-actin binding activity of cortactin. The acetylation level of cortactin may correlate to tumor metastases and acetylated cortactin may be considered a potential metastasis marker.

The antibody recognizes human acetylated Cortactin.

Epitope: Unknown
Consensus sequence motif of acetylation sites of cortactin.

Research Category
Cell Structure
Research Sub Category
Cytoskeleton
This Anti-acetyl Cortactin Antibody is validated for use in Western Blotting, for the detection of acetyl Cortactin.

Evaluated by Western Blot in HeLa-Anti-Cortactin immunocomplex treated with TSA/Nicotinamide.

Western Blot Analysis: 2.5 µg/ml of this antibody detected Cortactin on 10 µL of HeLa-Anti-Cortactin immunocomplex treated with TSA/Nicotinamide.

Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4, 150 mM NaCl) with 0.05% sodium azide.
Affinity purified

Control
HeLa-Anti-Cortactin immunocomplex treated with TSA/Nicotinamide

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

rabbit

100

affinity isolated antibody

primary antibodies

polyclonal

affinity chromatography

human, mouse

Drosophila (based on 100% sequence homology), rat (based on 100% sequence homology)

western blot: suitable

NP_005222.2

Q14247

wet ice

human ... CTTN(2017)

~ 85 kDa

Stable for 1 year at 2-8°C from date of receipt.

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

12 - Non Combustible Liquids

WGK 1