您的位置：首页 > 产品中心 > Anti-TKS5 (SH3 #1) Antibody
Anti-TKS5 (SH3 #1) Antibody
产品别名
Anti-TKS5 (SH3 #1) Antibody
Adaptor protein TKS5, Five SH3 domain-containing protein, SH3 and PX domains 2A, SH3 multiple domains 1, SH3 multiple domains protein 1, five SH3 domains
基本信息
| eCl@ss | 32160702 |
| NACRES | NA.41 |
| General description【一般描述】 | Tks5 (previously known as Fish), is a large scaffolding protein with one amino-terminal PX domain and five SH3 domains. It is cytoplasmic in normal fibroblasts. In Src-transformed cells, Tks5 localizes to podosomes. The coexpression of Tks5 and constitutively active Src in epithelial cancer cells resulted in the appearance of podosomes/invadopodia. Cells with reduced Tks5 expression are poorly invasive through matrigel. Tks5 is expressed and localized to podosomes/invadopodia in some invasive human cancer cell lines and tumor tissue, particularly breast cancers and melanomas. Thus, Tks5 appears to be required for podosome/invadopodia formation and for invasion of some cancer cells. |
| Specificity【特异性】 | Other homologies: Human, Canine, Equine, Rat, and Bovine (100% sequence homology), Chicken (93% sequence homology) |
| Immunogen【免疫原】 | Recombinant Protein, aa: 166-225. Homology: Human, Dog, Horse, Rat, Cow (100%, 60/60); Chicken (93%, 56/60) Epitope: 166-225 aa |
| Application【应用】 | Research Category Cell Structure Research Sub Category Cytoskeletal Signaling Western Blot Analysis: Antibody detected TKS5 in 20 μg of NIH-3T3 (100% confluent) lysate. Anti-TKS5 (SH3 #1) Antibody detects level of TKS5 (SH3 #1) & has been published & validated for use in WB, IC & IP. |
| Quality【质量】 | Routinely evaluated by Western Blot on NIH-3T3 (100% confluent) lysate. |
| Physical form【外形】 | Purified in 0.1M Tris-Glycine (pH7.4) 150mM NaCl with 0.05% NaN3. Format: Purified Protein A purified |
| Analysis Note【分析说明】 | Control Western Blot:: NIH-3T3 (100% confluent) lysate Immunocytochemistry: Mouse 3T3-Src(Y527F) cells |
| Other Notes【其他说明】 | Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration. |
产品性质
| biological source【生物来源】 | rabbit |
| Quality Level【质量水平】 | 100 |
| antibody form【抗体形式】 | purified antibody |
| antibody product type | primary antibodies |
| clone【克隆】 | polyclonal |
| species reactivity | human, mouse |
| technique(s) | immunocytochemistry: suitable immunoprecipitation (IP): suitable western blot: suitable |
| NCBI accession no.【NCBI登记号】 | NM_008018.3 |
| UniProt accession no.【UniProt登记号】 | O89032 |
| shipped in【运输】 | wet ice |
产品说明
| Target description【目标描述】 | 130-150 kDa Uncharacterized bands(s) may be observed in some cell lysates. |
| Storage and Stability【储存及稳定性】 | Maintain at 2-8°C in undiluted aliquots for up to 1 year after date of receipt. |
| Disclaimer【免责声明】 | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
安全信息
| Storage Class Code【储存分类代码】 | 12 - Non Combustible Liquids |
| WGK | WGK 1 |
