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Anti-Green Fluorescent Protein Antibody

产品编号:4090766
规格:Chemicon®, from rabbit
包装规格:50 μG
产品类别:进口试剂
品牌:Sigma-Aldrich
优惠价:立即咨询
产品价格
产品编号包装单位单价(元)国内现货国外库存询价单
409076650 μG5520
产品别名

Anti-Green Fluorescent Protein Antibody

GFP, eGFP

基本信息
eCl@ss
32160702
NACRES
NA.41
General description【一般描述】
The Green Fluorescent Protein (GFP) from the jellyfish Aequorea victoria is used as a fluorescent indicator for monitoring gene expression in a variety of cellular systems, including living organisms and fixed tissues. Unlike other bioluminescent reporters, GFP fluoresces in the absence of substrates, cofactors, or other intrinsic or extrinsic proteins. Purified GFP is a 27 kDa monomer consisting of 238 amino acids and emits green light (emission maximum at 509 nm) when excited with blue or UV light.
Specificity【特异性】
AB3080 is made against highly-purified native GFP from Aequorea victoria. It is reactive with GFP from both native and recombinant sources. While other commercial antibodies to GFP typically demonstrate strong reactivity with E. coli, the AB3080 GFP antibody has been purified by Protein A chromatography, followed by extensive adsorption against whole E. coli BL21 strain cells, giving them minimal cross-reactivity with E. coli.
Immunogen【免疫原】
Highly purified native GFP from Aequorea victoria.
Application【应用】
Research Category
Epitope Tags & General Use
Research Sub Category
Epitope Tags
Western Blot:
A previous lot of this antibody was used in western blot.

ELISA:
A 1:2000-1:10,000 dilution of a previous lot was used in Enzyme-linked Immunosorbent Assay.

Note: While AB3080 has been adsorbed against E. coli proteins, most secondary antibodies have not. To reduce the likelihood of E. coli reactivity from a secondary antibody, we suggest the use of Millipore’s highly-purified secondary antibodies in indirect detection systems.

Immunocytochemistry:

Optimal working dilutions must be determined by end user.
This Anti-Green Fluorescent Protein Antibody is validated for use in ELISA, IC, IH, WB for the detection of Green Fluorescent Protein.
Quality【质量】
Routinely evaluated by immunocytochemistry on RENCX cells.

Immunocytochemistry Analysis: Confocal fluorescent analysis of RENCX constitutively expressed GFP using this lot of anti-GFP Rabbit pAB (Red). Green indicates the expressed GFP.
Physical form【外形】
Format: Purified
Protein A purified
Purified rabbit polyclonal IgG in 10 mM Tris buffer, pH 7.2 with 0.01% sodium azide.
Analysis Note【分析说明】
Control
GFP fusion protein expressed in cells

RENCX cells
Other Notes【其他说明】
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Legal Information【法律信息】
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
产品性质
biological source【生物来源】
rabbit
Quality Level【质量水平】
100
antibody form【抗体形式】
affinity purified immunoglobulin
antibody product type
primary antibodies
clone【克隆】
polyclonal
species reactivity
vertebrates
manufacturer/tradename
Chemicon®
technique(s)
ELISA: suitable
immunocytochemistry: suitable
immunohistochemistry: suitable
western blot: suitable
UniProt accession no.【UniProt登记号】
P42212
shipped in【运输】
dry ice
产品说明
Target description【目标描述】
GFP is 27 kDa Dependent upon the molecular weight of the fusion protein being detected.
Storage and Stability【储存及稳定性】
Stable for 1 year at -20ºC from date of receipt.

Handling Recommendations:
Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Disclaimer【免责声明】
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
安全信息
Storage Class Code【储存分类代码】
12 - Non Combustible Liquids
WGK
WGK 1
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