您的位置:首页 > 产品中心 > Anti-Green Fluorescent Protein Antibody
Anti-Green Fluorescent Protein Antibody
产品别名
Anti-Green Fluorescent Protein Antibody
GFP, eGFP
基本信息
eCl@ss | 32160702 |
NACRES | NA.41 |
General description【一般描述】 | The Green Fluorescent Protein (GFP) from the jellyfish Aequorea victoria is used as a fluorescent indicator for monitoring gene expression in a variety of cellular systems, including living organisms and fixed tissues. Unlike other bioluminescent reporters, GFP fluoresces in the absence of substrates, cofactors, or other intrinsic or extrinsic proteins. Purified GFP is a 27 kDa monomer consisting of 238 amino acids and emits green light (emission maximum at 509 nm) when excited with blue or UV light. |
Specificity【特异性】 | Reactivity with other species has not been confirmed. AB3080P is made against highly-purified native GFP from Aequorea victoria. It is reactive with GFP from both native and recombinant sources. While other commercial antibodies to GFP typically demonstrate strong reactivity with E. coli, the AB3080P GFP antibody has been affinity purified, followed by extensive adsorption against whole E. coli BL21 strain cells, giving them minimal cross-reactivity with E. coli. |
Immunogen【免疫原】 | Highly purified native GFP from Aequorea victoria. |
Application【应用】 | Anti-Green Fluorescent Protein Antibody detects level of Green Fluorescent Protein & has been published & validated for use in ELISA, IC & WB. Research Category Epitope Tags & General Use Western Blot: A 1:500-1:1000 dilution of a previous lot was used using colorimetric detection (Peroxidase/TMB), with higher dilution possible using more sensitive detection methods. ELISA: A 1:800-1:1,000 dilution of a previous lot was used in ELISA. Note: While AB3080 has been adsorbed against E. coli proteins, most secondary antibodies have not. To reduce the likelihood of E. coli reactivity from a secondary antibody, we suggest the use of Millipore′s highly purified secondary antibodies in indirect detection systems. Immunocytochemistry: Optimal working dilutions and protocols must be determined by end user. Research Sub Category Epitope Tags |
Quality【质量】 | Routinely evaluated by immunocytochemistry on RENCX cells. Immunocytochemistry Analysis: Confocal fluorescent analysis of RENCX constitutively expressed GFP using this lot′s anti-GFP Rabbit pAB (Red). Green indicates the expressed GFP. |
Physical form【外形】 | Affinity purified rabbit polyclonal IgG in 10 mM Tris buffer. Affinity purified rabbit immunoglobulin |
Analysis Note【分析说明】 | Control Cells transfected with GFP-tagged fusion vector RENCX cells |
Other Notes【其他说明】 | Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration. |
Legal Information【法律信息】 | CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany |
产品性质
biological source【生物来源】 | rabbit |
Quality Level【质量水平】 | 100 |
antibody form【抗体形式】 | affinity purified immunoglobulin |
antibody product type | primary antibodies |
clone【克隆】 | polyclonal |
purified by【纯化方式】 | affinity chromatography |
species reactivity | vertebrates (most common) |
species reactivity (predicted by homology) | rat |
manufacturer/tradename | Chemicon® |
technique(s) | ELISA: suitable immunocytochemistry: suitable western blot: suitable |
UniProt accession no.【UniProt登记号】 | P42212 |
shipped in【运输】 | dry ice |
产品说明
Target description【目标描述】 | Varies (GFP is 27 kDa) |
Storage and Stability【储存及稳定性】 | Stable for 1 year at -20°C in undiluted aliquots from date of receipt. Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. |
Disclaimer【免责声明】 | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
安全信息
Storage Class Code【储存分类代码】 | 12 - Non Combustible Liquids |
WGK | nwg |