广州伟伯科技有限公司
您的位置:首页 > 产品中心 > Tumor Necrosis Factor-α, mouse (mTNF-α)
产品搜索:

Tumor Necrosis Factor-α, mouse (mTNF-α)

产品编号:4090825
规格:recombinant (E. coli)
包装规格: 2000000 UNITS
产品类别:进口试剂
品牌:Roche
优惠价:立即咨询
产品价格
产品编号包装单位单价(元)国内现货国外库存询价单
4090825 2000000 UNITS5340
产品性质
Quality Level【质量水平】
100
biological source【生物来源】
mouse
recombinant【重组】
expressed in E. coli
Assay【测定】
>95% (SDS-PAGE)
form【形式】
solution
potency【效能】
<0.0025 ng/mL EC50
mol wt【分子量】
17,200 Da
packaging【包装】
pkg of 2,000,000 U (5 μg, 1ml)
manufacturer/tradename
Roche
storage condition【储存条件】
avoid repeated freeze/thaw cycles
impurities【杂质】
<1 EU/mgtested (LAL test)
UniProt accession no.【UniProt登记号】
Q6TDG3
shipped in【运输】
dry ice
storage temp.【储存温度】
−20℃
Gene Information
mouse ... Tnf(21926)
基本信息
General description【一般描述】
Recombinant murine TNF-α is produced in E. coli and purified by standard chromatographic techniques.
Contents:
5 μg/ml solution in PBS and 1 mg/ml BSA, filtered through a 0.2 μm pore size membrane
Specificity【特异性】
Mouse mTNF-α is effective on mouse, rat, rabbit, and human cells.
Specific activity/EC50: >4 x 108 U/mg, <0.0025 ng/ml (mTNF-a, NIBSC, interim standard, 88/532), at least the same specific activity (EC50) compared to the indicated standard is guaranteed.
Application【应用】
TNF-α causes selective necrosis of murine tumors when injected into tumor-bearing mice. In vitro, human TNF-α has direct cytolytic or cytostatic activity on certain transformed cells. In this context, it acts synergistically with interferon-γ. It has also been used for cell stimulation studies.
Quality【质量】
Purity: >95% (SDS-PAGE)
Endotoxin level: <0.1 EU/μg (LAL-test), <10 EU/ml (LAL-test)
Note: 1 EU corresponds to 0.1 ng
Specifications【产品规格】
Primary structure: One polypeptide chain (156 amino acids) identical to natural mTNF-a, but not glycosylated. Glycosylation is not essential for biological activity.
Sequence【序列】
Chain Length 156 AA
One polypeptide chain (156 amino acids) identical to natural mTNF-α, but not glycosylated. Glycosylation is not essential for biological activity.
Unit Definition【单位定义】
EC50 definition: The amount of mTNF-α that is required to mediate half-maximal cytotoxicity (MTT cleavage) with WEHI 164 cells in the presence of actinomycin D (1 unit equals ≤0.0025 ng/ml).
Preparation Note【制备说明】
Storage conditions (working solution): -15 to -25 ℃
The solution can be stored in aliquots at -15 to -25 ℃.
Note: Avoid repeated freezing and thawing.
Other Notes【其他说明】
仅用于生命科学研究。不可用于诊断。
安全信息
Storage Class Code【储存分类代码】
12 - Non Combustible Liquids
WGK
nwg
广州伟伯科技有限公司 版权所有 CopyRight ©2006-2024, All Rights Reserved
工信部备案号:粤ICP备08114744号   Page Run Time: 0.0087