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Anti-α-Tubulin Mouse mAb (DM1A)
产品性质
| Quality Level【质量水平】 | 100 |
| biological source【生物来源】 | mouse |
| antibody form【抗体形式】 | purified antibody |
| antibody product type | primary antibodies |
| clone【克隆】 | DM1A, monoclonal |
| form【形式】 | liquid |
| contains【包含】 | ≤0.1% sodium azide as preservative |
| species reactivity | human, gerbil, rat, chicken, mouse |
| manufacturer/tradename | Calbiochem® |
| storage condition【储存条件】 | do not freeze |
| isotype【同位素/亚型】 | IgG1 |
| shipped in【运输】 | wet ice |
| storage temp.【储存温度】 | 2-8℃ |
| packaging【包装】 | Please refer to vial label for lot-specific concentration. |
基本信息
| General description【一般描述】 | This Anti-α-Tubulin Mouse mAb (DM1A) is validated for use in Immunoblotting, Immunofluorescence for the detection of α-Tubulin. Purified mouse monoclonal antibody generated by immunizing Balb/c57 mice with the specified immunogen and fusing splenocytes with NS-1 mouse myeloma cells. Recognizes the ~60 kDa tubulin protein. Recognizes the ~60 kDa tubulin protein found in all eukaryotic cells. May be used as a positive control. |
| Immunogen【免疫原】 | Chicken native chick brain microtubules |
| Application【应用】 | Immunoblotting (20-100 ng/ml, colorimetric or chemiluminescence; see application references) Immunofluorescence (5 µg/ml) |
| Warning【警告】 | Toxicity: Standard Handling (A) |
| Physical form【外形】 | In 50 mM sodium phosphate buffer, 0.2% gelatin. |
| Analysis Note【分析说明】 | Positive Control Any cell line Negative Control trp E (Ab-1) |
| Other Notes【其他说明】 | Tubulin is found in all cells and is used as a positive control for immunofluorescence or immunoblotting. Antibody should be titrated for optimal results in individual systems. Blose, S.H., et al. 1984. J. Cell. Biol.98, 847. Lin, J.J.C., et al. 1984. In: Monoclonal antibodies and functional cell lines. Kennett, R.H., Bechtol, K.B. and McKearn, T.J., eds. Plenum Publishing Corp. Lin, J.J.C. 1982. Cold Spring Harbor Symp. on Quant. Biol.46, 769. Goldman, R.D., et al. 1979. Ann. Rev. Physiol.41, 701. Goldman, R., Pollard, T. and Rosenbaum, J. (eds.) 1976. Cold Spring Harbor Conference on Cell Proliferation, Books A-C, Cold Spring Harbor, New York. |
| Legal Information【法律信息】 | CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany |
安全信息
| Storage Class Code【储存分类代码】 | 11 - Combustible Solids |
| WGK | WGK 1 |
