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布氏肉汤基础
Brucella broth base
| 产品编号： | 4091036 |
| 规格： | suitable for microbiology, NutriSelect® Plus |
| 包装规格： | 500 G |
| 产品类别： | 进口试剂 |
| 品牌： | Millipore |
| 优惠价： | 立即咨询 |
产品别名
布氏肉汤基础
Brucella broth base
基本信息
| NACRES | NA.85 |
| Application【应用】 | 推荐用于 Brucella 菌种的增菌、分离和培养。 |
| Components【组分】 | Components (g/L): Casein Enzymic Hydrolysate 10.00 Peptic Digest of Animal Tissue 10.00 Yeast Extract 2.00 Dextrose 1.00 Sodium Chloride 5.00 Sodium bisulphite 0.10 |
| Preparation Note【制备说明】 | Suspend 14.05 g of Brucella Broth Base in 500 ml of distilled water. Heat to boiling to dissolve the medium completely. Sterilize by autoclaving at 15 lbs. pressure (121℃) for 15 minutes. Cool to 45-50℃ and aseptically add sterile 5% v/v heat inactivated (H1138) horse serum. Add polymyxin B (2,500 IU, Cat. No. 81334), bacitracin (12,500 IU, Cat. No. 11702), nystatin (50,000 IU, Cat. No. 74721), cycloheximide (50mg, Cat. No. 01810), nalidixic acid (2.5mg, Cat. No. 70162) and vancomycin (10mg, Cat. No. 94747) to make the medium selective. Mix well before pouring into sterile petri plates or tubes. For isolation of the Campylobacter species add the rehydrated contents of one vial of Campylobacter Growth Supplement (Cat. No. C2847) and 5-7% defibrinated sheep blood to 500 ml of medium base. 500 ml of medium base. The Campylobacter Growth Supplement can also be made by mixing 125 mg sodium pyruvate (Cat. No. 15990), 125 mg sodium metabisulfite (Cat. No. 71928 )and 125 mg ferrous sulfate (Cat. No. 44970) in 2 ml of distilled water and filtering the solution through a sterile 0.4 mm filter to sterilize. |
| Footnote【附注】 | We offer two media types: the superior granulated GranuCult® and the cost-efficient powdered NutriSelect® culture media, depending on your needs. The designations basic, plus, or prime are added to indicate the quality control level, from basic quality control to standard QC plus to prime for full regulatory compliance. |
| Legal Information【法律信息】 | GRANUCULT is a registered trademark of Merck KGaA, Darmstadt, Germany NutriSelect is a registered trademark of Merck KGaA, Darmstadt, Germany |
产品性质
| Quality Level【质量水平】 | 200 |
| sterility【无菌性】 | non-sterile |
| form【形式】 | powder |
| shelf life【保质期】 | limited shelf life, expiry date on the label |
| manufacturer/tradename | NutriSelect® Plus |
| technique(s) | microbiological culture: suitable |
| pH【pH值(酸碱度)】 | 7.0±0.2 (25 ℃) |
| application(s) | clinical testing environmental food and beverages sample preparation microbiology |
| suitability【适用性】 | selective for Brucella spp. selective for Campylobacter spp. |
安全信息
| Storage Class Code【储存分类代码】 | 11 - Combustible Solids |
| WGK | WGK 3 |
| Personal Protective Equipment【个人防护装备】 | Eyeshields, Gloves, type N95 (US) |
