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Anti-Neurofilament 200kDa Antibody, clone NE14, Biotin Conjugate
| 产品编号： | 4091083 |
| 规格： | clone NE14, from mouse, biotin conjugate |
| 包装规格： | 100 μL |
| 产品类别： | 进口试剂 |
| 品牌： | Sigma-Aldrich |
| 优惠价： | 立即咨询 |
产品别名
Anti-Neurofilament 200kDa Antibody, clone NE14, Biotin Conjugate
Neurofilament heavy polypeptide, NF-H, 200 kDa neurofilament protein, Neurofilament triplet H protein
基本信息
| eCl@ss | 32160702 |
| NACRES | NA.41 |
| General description【一般描述】 | Neurofilaments are a type of intermediate filament that serve as major elements of the cytoskeleton supporting the axon cytoplasm. They are the most abundant fibrillar components of the axon, being on average 3-10 times more frequent than axonal microtubules. Neurofilaments (10nm in dia.) are built from three intertwined protofibrils which are themselves composed of two tetrameric protofilament complexs of monomeric proteins. The neurofilament triplet proteins (68/70, 160, and 200 kDa) occur in both the central and peripheral nervous system and are usually neuron specific. The 68/70 kDa NF-L protein can self-assemble into a filamentous structure, however the 160 kDa NF-M and 200 kDa NF-H proteins require the presence of the 68/70 kDa NF-L protein to co-assemble. Neuromas, ganglioneuromas, gangliogliomas, ganglioneuroblastomas and neuroblastomas stain positively for neurofilaments. Although typically restricted to neurons, neurofilaments have been detected in paragangliomas and adrenal and extra-adrenal pheochromocytomas. Carcinoids, neuroendocrine carcinomas of the skin, and oat cell carcinomas of the lung also express neurofilaments. For more neurofilament information and epitope maps, search "neurofilament" on our website. |
| Immunogen【免疫原】 | Purified human neurofilament polypeptides (Debus et al., 1983). |
| Application【应用】 | Research Category Neuroscience Immunohistochemistry Analysis: A 1:100 dilution from a representative lot detected Neurofilament in adult mouse brain cryosections. Anti-Neurofilament 200kDa Antibody, clone NE14, Biotin Conjugate detects level of Neurofilament 200kDa & has been published & validated for use in ICC & IHC. Research Sub Category Neurofilament & Neuron Metabolism |
| Quality【质量】 | Evaluated by Immunocytochemistry in rat E18 primary cortex cells. Immunocytochemistry Analysis: A 1:100 dilution of this antibody detected Neurofilament in rat E18 primary cortex cells. |
| Physical form【外形】 | Purified mouse monoclonal IgG1 conjugated to Biotin in PBS with 0.1% sodium azide and 15 mg/mL BSA. Protein A purified |
| Analysis Note【分析说明】 | Control Rat E18 primary cortex cells |
产品性质
| Quality Level【质量水平】 | 100 |
| biological source【生物来源】 | mouse |
| conjugate【偶联物】 | biotin conjugate |
| antibody form【抗体形式】 | purified immunoglobulin |
| antibody product type | primary antibodies |
| clone【克隆】 | NE14, monoclonal |
| species reactivity | rat, mouse, pig |
| species reactivity (predicted by homology) | human (based on 100% sequence homology), porcine (based on 100% sequence homology) |
| technique(s) | immunocytochemistry: suitable immunohistochemistry: suitable |
| isotype【同位素/亚型】 | IgG1 |
| NCBI accession no.【NCBI登记号】 | NP_066554 |
| UniProt accession no.【UniProt登记号】 | P12036 |
| shipped in【运输】 | wet ice |
产品说明
| Storage and Stability【储存及稳定性】 | Stable for 1 year at 2-8°C from date of receipt. |
| Disclaimer【免责声明】 | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
安全信息
| Storage Class Code【储存分类代码】 | 12 - Non Combustible Liquids |
| WGK | WGK 2 |
