您的位置：首页 > 产品中心 > LIGHT human
LIGHT human
| 产品编号： | 4091276 |
| 规格： | recombinant, expressed in Hi-5 Insect cells, ≥96% (SDS-PAGE), ≥96% (HPLC), suitable for cell culture |
| 包装规格： | 15 μG |
| 产品类别： | 进口试剂 |
| 品牌： | Sigma-Aldrich |
| 优惠价： | 立即咨询 |
产品别名
LIGHT human
HVEM-L
TNFSF14
基本信息
| NACRES | NA.32 |
| General description【一般描述】 | LIGHT belongs to the TNF family of ligands, and can signal through the herpes virus entry mediator type A receptor (HVEM, TNFRSF14), LTbR, or bind to a decoy receptor, DcR3. It is expressed in splenocytes, activated PBL, CD+8 tumor infiltrating lymphocytes, granulocytes, and monocytes. LIGHT has the ability to active NFkB, to co-stimulate the activation of lymphocytes and to induce apoptosis in certain human tumor cells. Recombinant human LIGHT has a calculated mass of 19.3 kDa containing 177 amino acid residues. Due to glycosylation LIGHT migrates between 20.0-22.5 kDa by SDS-PAGE under non-reducing conditions. |
| Biochem/physiol Actions【生化/生理作用】 | LIGHT belongs to the TNF family of ligands, and can signal through the herpes virus entry mediator type A receptor (HVEM, TNFRSF14), LTbR, or bind to a decoy receptor, DcR3. Recombinant human LIGHT has a calculated mass of 19.3 kDa containing 177 amino acid residues. Due to glycosylation LIGHT migrates between 20.0-22.5 kDa by SDS-PAGE under non-reducing conditions. |
| Sequence【序列】 | RLGEMVTRLP DGPAGSWEQL IQERRSHEVN PAAHLTGANS SLTGSGGPLL WETQLGLAFL RGLSYHDGAL VVTKAGYYYI YSKVQLGGVG CPLGLASTIT HGLYKRTPRY PEELELLVSQ QSPCGRATSS SRVWWDSSFL GGVVHLEAGE KVVVRVLDER LVRLRDGTRS YFGAFMV |
| Physical form【外形】 | Lyophilized from 20 mM Sodium Phosphate, pH 7.0. |
| Reconstitution【重悬】 | Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/ml. Do not vortex. This solution can be stored at 2-8℃ for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20℃ to -80℃. |
产品性质
| biological source【生物来源】 | human |
| recombinant【重组】 | expressed in Hi-5 Insect cells |
| Assay【测定】 | ≥96% (HPLC) ≥96% (SDS-PAGE) |
| form【形式】 | lyophilized |
| mol wt【分子量】 | 20.0-22.5 kDa |
| packaging【包装】 | pkg of 15 μg |
| technique(s) | cell culture | mammalian: suitable |
| impurities【杂质】 | <0.1 EU/μg endotoxin, tested |
| color【颜色】 | white to off-white |
| UniProt accession no.【UniProt登记号】 | O43557 |
| shipped in【运输】 | wet ice |
| storage temp.【储存温度】 | −20℃ |
| Gene Information | human ... TNFSF14(8740) |
安全信息
| Storage Class Code【储存分类代码】 | 13 - Non Combustible Solids |
| WGK | WGK 3 |
