CDK6/CyclinD1, active, His/GST tagged human

200

expressed in baculovirus infected Sf9 cells

PRECISIO^® Kinase

≥70% (SDS-PAGE)

buffered aqueous glycerol solution

7.9-10.7 nmol/min·mg

CDK6 subunit ~40 kDa
cyclin D1 ~61 kDa

P24385
Q00534

dry ice

−70℃

human ... CCND1(595), CDK6(1021)

CDK6 is a member of the cyclin-dependent family of protein kinases that are important regulators of cell cycle progression. CDK6 activity is regulated by the D-type cyclins and members of the INK4 family of CDK inhibitors. The CDK6 kinase activity is detected in mid-G1 phase of the cell cycle and is responsible for the phosphorylation and regulation of the activity of tumor suppressor protein Rb. Although CDK6 and CDK4 can both phosphorylate multiple residues in the Rb protein, they do so with different residue selectivity in vitro; CDK6 phosphorylates Thr821 while CDK4 phosphorylates Thr826 on Rb protein.

Supplied in 50 mM Sodium Phosphate, pH 7.0, with 300 mM NaCl, 150 mM imidazole, 0.1 mM PMSF, 0.2 mM DTT, and 25% glycerol.

PRECISIO is a registered trademark of sigma-aldrich Co. LLC

10 - Combustible liquids

WGK 1