Anti-Cre Recombinase Antibody, clone 2D8

32160702

NA.41

Cre-recombinase is a 38 kDa DNA recombinase derived from the P1 bacteriophage. It is highly specific for a 34 bp DNA sequence (loxP) found in P1 DNA. It catalyzes site-specific recombination between two 34-base-pair LOXP sites. Its role is to maintain the phage genome as a monomeric unit-copy plasmid in the lysogenic state. It is homotetramer when bound to DNA and belongs to the "phage" integrase family. This antibody can be used to determine the success of gene manipulations in transgenic animals.

Cre recombinase. The epitope is located between aa 77 and 343.

Cre recombinase (aa 77 to 343) fusion protein

Research Sub Category
Cell Cycle, DNA Replication & Repair
Western Blot (natural and recombinant protein):
A 1:500-1:5,000 dilution was used in a previous lot. Natural Cre recombinase from Bacteriophage P1 migrates at 38.5 kDa by SDS-PAGE.

Immunofluorescence:
A previous lot of this antibody was used on IF.

Immunohistochemistry:
A 1:500-1:5,000 dilution of a previous lot was used in IH.

ELISA:
A 1:500-1:5,000 dilution of a previous lot was used in ELISA.

Optimal working dilutions must be determined by the end user.
Anti-Cre Recombinase Antibody, clone 2D8 is a well characterized monoclonal antibody. Proven to reliably detect Cre Recombinase, this mAb is validated for use in ELISA, IC, IF, IH & WB and is backed by multiple publications.
Research Category
Epigenetics & Nuclear Function

Ascites fluid. Contains no preservative
Unpurified

Control
HeLa Nuclear Extract

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

100

mouse

ascites fluid

primary antibodies

2D8, monoclonal

all

Chemicon^®

ELISA: suitable
immunocytochemistry: suitable
immunofluorescence: suitable
immunohistochemistry: suitable
western blot: suitable

IgG1κ

P06956

dry ice

38 kDa

Stable for 1 year at -20ºC from date of receipt.

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

10 - Combustible liquids

WGK 1