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Anti-phospho-SMAD3 (Tyr179) Antibody
| 产品编号： | 4092083 |
| 规格： | from rabbit, purified by affinity chromatography |
| 包装规格： | 100 μL |
| 产品类别： | 进口试剂 |
| 品牌： | Sigma-Aldrich |
| 优惠价： | 立即咨询 |
产品别名
Anti-phospho-SMAD3 (Tyr179) Antibody
Mothers against DPP homolog 3, MAD homolog 3, Mad3, Mothers against decapentaplegic homolog 3, JV15-2, AMAD3, SMAD family member 3
基本信息
| eCl@ss | 32160702 |
| NACRES | NA.41 |
| General description【一般描述】 | Members of the Smad family of signal transduction molecules are components of a critical intracellular pathway that transmits TGF-β signals from the cell surface into the nucleus. Three distinct classes of Smads have been defined: the receptor-regulated Smads (R-Smads), which include Smad1, 2, 3, 5 and 8, the common-mediator Smad (co-Smad), Smad4, and the antagonistic or inhibitory Smads (I-Smads), Smad6 and 7. Once in the nucleus, Smads can target a variety of DNA binding proteins to regulate transcriptional responses. Following stimulation by TGF-β, Smad2 and Smad3 become phosphorylated at their carboxyl termini (Ser465 and 467 on Smad2; Ser423 and 425 on Smad3) by TGF-β Receptor I. Phosphorylated Smad 2/3 can complex with Smad4, translocate to the nucleus and regulate gene expression. |
| Specificity【特异性】 | Other homologies: Zebrafish (91% sequence homology), Chicken (91% sequence homology) and Xenopus (91% sequence homology). This antibody recognizes the Linker region of SMAD3 phosphorylated at Thr179. |
| Immunogen【免疫原】 | Epitope: Linker region KLH-conjugated linear peptide corresponding to the Linker Region of human SMAD3 phosphorylated at Tyr179.. |
| Application【应用】 | Research Category Signaling Detect phospho-SMAD3 (Tyr179) using this Anti-phospho-SMAD3 (Tyr179) Antibody validated for use in Western Blotting. Research Sub Category Developmental Signaling |
| Quality【质量】 | Evaluated by Western Blotting in untreated and TGF Beta-treated HaCat cell lysates. Western Blotting Analysis: A 1:1,000 dilution of this antibody detected SMAD3 in 10 mg of untreated and TGF Beta-treated HaCat cell lysates. |
| Physical form【外形】 | Purified rabbit polyclonal serum in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.5% sodium azide. Affinity purified |
| Analysis Note【分析说明】 | Control Untreated and TGF Beta-treated HaCat cell lysates. |
| Other Notes【其他说明】 | Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration. |
产品性质
| biological source【生物来源】 | rabbit |
| Quality Level【质量水平】 | 100 |
| antibody form【抗体形式】 | affinity isolated antibody |
| antibody product type | primary antibodies |
| clone【克隆】 | polyclonal |
| purified by【纯化方式】 | affinity chromatography |
| species reactivity | human |
| species reactivity (predicted by homology) | bovine, rat, porcine |
| technique(s) | western blot: suitable |
| NCBI accession no.【NCBI登记号】 | NP_005893 |
| UniProt accession no.【UniProt登记号】 | P84022 |
| shipped in【运输】 | wet ice |
产品说明
| Target description【目标描述】 | ~52 kDa observed. Uncharacterized bands at ~170 kDa, ~165 kDa, ~90 kDa and ~52 kDa may be observed in untreated HaCat cell lysate and in some other cell lysates. Uncharacterized bands at ~105 kDa and ~55 kDa may be observed in some cell lysates. |
| Storage and Stability【储存及稳定性】 | Stable for 1 year at 2-8º from date of receipt. |
| Disclaimer【免责声明】 | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
安全信息
| Storage Class Code【储存分类代码】 | 12 - Non Combustible Liquids |
| WGK | WGK 1 |
