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基本信息
| NACRES | NA.81 |
| General description【一般描述】 | Dermal Fibroblasts are responsible for producing the extracellular matrix forming the connective tissue of the skin, and play a crucial role during wound healing. HDF provide an excellent model system to study many aspects of cell physiology, and have been utilized in dozens of research publications, particularly those related to skin biology and reprogramming/induced pluripotency studies. HDF were used to create, along with human synovyocytes, induced pluripotent stem cells (iPSC) by using the now classic “Yamanaka cocktail” of Oct3/4, Sox2, Klf4, and c-Myc, the discovery for which Dr. Shinya Yamanaka was awarded the Nobel Prize in 2012 (Takanashi, 2007; 2009), and to characterize iPSC general properties, miRNA expression profiles and differentiation potential (Hayashi, 2010; Ohta, 2011; Koyanagi-Aoi, 2013; Noguchi, 20130; Razak, 2013; Sakurai, 2013; Tanaka, 2013; Yanagida, 2013) |
| Cell Line Origin【细胞系来源】 | Skin |
| Application【应用】 | injury recovery, connective tissue research, laminin and fibronectin production, extracellular matrix, tissue organization, wound healing, fibroblasts growth factor response |
| Components【组分】 | Fibroblast Basal Medium containing 10% FBS & 10% DMSO |
| Preparation Note【制备说明】 |
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| Subculture Routine【传代培养常规】 | Please refer to the HDF Culture Protocol. |
产品性质
| Quality Level【质量水平】 | 100 |
| biological source【生物来源】 | human neonatal foreskin or adult skin (normal) |
| packaging【包装】 | pkg of 500,000 cells |
| growth mode【生长模式】 | Adherent |
| karyotype【核型】 | 2n = 46 |
| morphology【形态学】 | Fibroblast |
| technique(s) | cell culture | mammalian: suitable |
| shipped in【运输】 | dry ice |
| storage temp.【储存温度】 | −196℃ |
安全信息
| Storage Class Code【储存分类代码】 | 13 - Non Combustible Solids |
| WGK | WGK 3 |
