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Anti-EGFR Antibody, clone 528 (Azide-free)
产品别名
Anti-EGFR Antibody, clone 528 (Azide-free)
Epidermal growth factor receptor, Proto-oncogene c-ErbB-1, Receptor tyrosine-protein kinase erbB-1
基本信息
| eCl@ss | 32160702 |
| NACRES | NA.41 |
| General description【一般描述】 | The epidermal growth factor receptor (EGFR; Proto-oncogene c-ErbB-1) is a ubiquitously receptor tyrosine kinase that is activated by a variety of ligands including EGF, EPGN, TGFA, and EREG. Ligand-bound EGFR undergoes dimerization and autophosphorylation exposing cytoplasmic domains which allow interaction with adaptor proteins and induction of several signaling pathways including PI3-AKT; PLCγ-PKC; Notch1; and Ras-Raf. EGFR therefore contributes to a diverse range of cellular processes inlcuding cell proliferation. Several studies have reported that EGFR signaling plays an important role in the development of various tumors. EGFR is regulated by PTPRJ and PTPRK phosphatases, and by the endocytic machinery including EPS15 that mediates internalization and degradation of the receptor. |
| Specificity【特异性】 | This antibody recognizes the extracellular domain of EGFR. |
| Immunogen【免疫原】 | Partially purified human EGFR from A431 cells |
| Application【应用】 | Neutralizing Assay Analysis: A representative lot was used by an independent laboratory in A431 cells. (Kawamoto, T., et al. (1984). Journal of Biological Chemistry. 259(12):7761-7766.) Anti-EGFR Antibody, clone 528 (Azide-free) detects level of EGFR & has been published & validated for use in Immunoprecipitation, Neutralizing. |
| Quality【质量】 | Evaluated by Immunoprecipitation in A431 cell lysate. Immunoprecipitation Analysis: 10 µg of this antibody immunoprecipitated EGFR from A431 cell lysate. |
| Linkage【联系】 | Replaces: 04-338 |
| Physical form【外形】 | Protein G Purified Format: Purified |
| Other Notes【其他说明】 | Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration. |
产品性质
| Quality Level【质量水平】 | 100 |
| biological source【生物来源】 | mouse |
| antibody form【抗体形式】 | purified antibody |
| antibody product type | primary antibodies |
| clone【克隆】 | 528, monoclonal |
| species reactivity | human |
| technique(s) | immunoprecipitation (IP): suitable neutralization: suitable |
| isotype【同位素/亚型】 | IgG2aκ |
| NCBI accession no.【NCBI登记号】 | NP_005219 |
| UniProt accession no.【UniProt登记号】 | P00533 |
| shipped in【运输】 | dry ice |
产品说明
| Target description【目标描述】 | ~170 kDa observed. Uniprot gives a calculated molecular weight of 135 but can be observed at ~170 kDa due to phosphorylation. (Konishi, A., et al. (2003). The Journal of Biological Chemistry. 278(37):35049–35056.) |
| 【免责声明】 | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
安全信息
| Storage Class Code【储存分类代码】 | 12 - Non Combustible Liquids |
| WGK | WGK 2 |
