Anti-Integrin β1 Antibody, clone N29 (Azide Free)

32160702

NA.41

Reacts with the human integrin beta1 subunit. Specificity verified by preclearing of beta1in immunoprecipitation, flow cytometry on transfectant cells displaying human beta1 integrin, and reactivity with purified beta1. Recognizes an epitope cluster distinct from MAB2250 and MAB2251(Wilkins et al., 1996).

Jurkat T-leukemic cell line

Research Category
Cell Structure
This Anti-Integrin β1 Antibody, clone N29 (Azide Free) is validated for use in Flow Cytometry and Immunohistochemistry and Immunoprecipitation and Affects Function and Cell Culture and Western Blotting for the detection of Integrin β1.
Research Sub Category
Integrins
Immunohistochemistry (Paraffin) Analysis: A 1:50-2,000 dilution from a representative lot detected Integrin β1 in the membrane of tubule epithelial cells and cells of the glomeruli in human kidney tissue, membrane of alveolar cells, endothelial cells, and bronchiole epithelial cells of human lung tissue.

Immunoprecipitation: A representative lot of this antibody clone was used in immunoprecipitation.

Immunohistochemistry: A representative lot of this antibody clone was used in immunohistochemistry on frozen sections.

Flow Cytometry: A representative lot of this antibody clone was used in flow cytometry.

Functional Activity Assay: A representative lot of this antibody clone was used to stimulate adhesion of cells to extracellular matrix proteins (Wilkins, J.A. et al., 1996).

Optimal working dilutions must be determined by end user.

Western Blot Analysis: 0.5 µg/mL of the antibody detected Integrin β1 in 10 µg of U251 cell lysate.

Format: Purified
Protein A purified
Purified cultured supernatant in PBS without preservatives.

Control
Tonsil, human skin, human kidney tissue

U251 cell lysate

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

100

mouse

culture supernatant

primary antibodies

N29, monoclonal

human

Chemicon^®

cell culture | mammalian: suitable
flow cytometry: suitable
immunohistochemistry: suitable
immunoprecipitation (IP): suitable
western blot: suitable

IgGκ

NM_002292.3

P55268

wet ice

88 kDa

Store at -20°C in undiluted aliquots.
Avoid repeated freeze-thaw cycles.

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

12 - Non Combustible Liquids

WGK 1