Rat/Mouse Ghrelin (total) ELISA

32161000

NA.32

Circulating ghrelin is a multifunctional hormone secreted primarily by the stomach. Originally found as an endogenous ligand for the growth hormone secretagogue receptor, it is also an appetite-stimulating hormone in the regulation of energy homeostasis. It consists of 28 amino acids and the n-octanoylation of serine-3 position is necessary for its bioactivity. The acylated form is extremely unstable in the blood; consequently, the predominant form is des-octanoyl ghrelin. Long considered a key player in obesity, ghrelin may also play a role in maintaining the balance between insulin and glucose and the development of diabetes. The spike in insulin secretion occurring after eating usually correlates with a dip in ghrelin production. Researchers determined that in rat models of uncontrolled diabetes, food intake still suppressed ghrelin concentrations in the blood, but only approximately half as effectively as when insulin was present.

Canine Ghrelin (Active) - 71%
Human Ghrelin (Active) - 115%
Human Des-Octanoyl Ghrelin - 249%
Porcine Ghrelin (Active) - 48%
Rat/Mouse Ghrelin (Active) - 85%
Rat/Mouse Des-Octanoyl Ghrelin - 100%

Room temperature, 3 hour assay, 20 μL sample size (serum or plasma)
Research Sub Category
Obesity

Metabolic Disorders
Research Category
Metabolism
Used to detect/quantify: Ghrelin (Total)

Please contact Technical Service for linearity of dilution.

100

rat, mouse

accuracy: 80-103%
(Serum: Active Ghrelin)
accuracy: 86-102%
(Plasma: Active Ghrelin)
accuracy: 92-103%
(Serum: Des-octanoyl Ghrelin)
accuracy: 95-107%
(Plasma: Des-octanoyl Ghrelin)
inter-assay cv: 1.8-4.5%
intra-assay cv: 0.7-1.3%
(Serum)
inter-assay cv: 2.0-3.2%
intra-assay cv: 1.1-1.7%
(Plasma)

ELISA: suitable

Q9EQX0 (mouse)
Q9QYH7 (rat)
Q9UBU3

colorimetric
fluorometric

For research use only. Not for use in diagnostic procedures.

GHS05,GHS06,GHS09

Danger

H290 - H302 + H332 - H311 - H315 - H317 - H319 - H335 - H411

P273 - P280 - P301 + P312 - P302 + P352 + P312 - P304 + P340 + P312 - P305 + P351 + P338

Acute Tox. 3 Dermal - Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Aquatic Chronic 2 - Eye Irrit. 2 - Met. Corr. 1 - Skin Irrit. 2 - Skin Sens. 1 - STOT SE 3

Respiratory system

6.1C - Combustible, acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects

WGK 3