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Anti-Ki-67 Antibody
基本信息
eCl@ss | 32160702 |
NACRES | NA.41 |
General description【一般描述】 | Ki67 antigen is the prototypic cell cycle related nuclear protein, expressed by proliferating cells in all phases of the active cell cycle (G1, S, G2 and M phase). It is absent in resting (G0) cells. Ki67 antibodies are useful in establishing the cell growing fraction in neoplasms (immunohistochemically quantified by determining the number of Ki67 positive cells among the total number of resting cells = Ki67 index). In neoplastic tissues the prognostic value is comparable to the tritiated thymidine labelling index. The correlation between low Ki67 index and histologically low grade tumours is strong. Ki67 is routinely used as a neuronal marker of cell cycling and proliferation. |
Specificity【特异性】 | Recognizes Ki-67 |
Immunogen【免疫原】 | Synthetic peptide from human Ki-67 |
Application【应用】 | Detect Ki-67 with Anti-Ki-67 Antibody (Rabbit Polyclonal Antibody), that has been shown to work in IHC(P) & WB. Immunocytochemistry: Confolcal fluorescent analysis of A431 cells using AB9260 rabbit pAB. Actin filaments were labeled with Alexa Fluor 488 -Phalloidin. Nuclear is stained with DAPI. (Nuclear staining). Western Blot Analysis: A previous lot of this antibody was used in western blot. Optimal working dilutions must be determined by the end user. |
Quality【质量】 | Immunohistochemistry(paraffin): Representative lot data. This antibody was used on formalin fixed, paraffin embedded tissue at 1:300 dilution for 10 minutes at room temperature. Staining of formalin-fixed tissues requires boiling of tissues in 10 mM citrate buffer, pH 6.0 for 10 minutes followed by cooling at room temperature for 20 minutes. |
Physical form【外形】 | ImmunoAffinity Purified Purified rabbit polyclonal in buffer containing 10 mM PBS, pH 7.4 containing BSA and sodium azide. |
Analysis Note【分析说明】 | Control Positive Control: Breast carcinoma, lymph node tissue, normal tonsil. |
Other Notes【其他说明】 | Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration. |
Legal Information【法律信息】 | CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany |
产品性质
biological source【生物来源】 | rabbit |
Quality Level【质量水平】 | 100 |
antibody form【抗体形式】 | affinity isolated antibody |
antibody product type | primary antibodies |
clone【克隆】 | polyclonal |
purified by【纯化方式】 | affinity chromatography |
species reactivity | human, mouse |
species reactivity (predicted by homology) | rat |
manufacturer/tradename | Chemicon® |
technique(s) | immunohistochemistry: suitable (paraffin) western blot: suitable |
NCBI accession no.【NCBI登记号】 | NM_002417.3 |
UniProt accession no.【UniProt登记号】 | P46013 |
shipped in【运输】 | wet ice |
产品说明
Target description【目标描述】 | Predicted: 359 kDa MW may be observed at ~345 or 395 kDa |
【免责声明】 | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
安全信息
Storage Class Code【储存分类代码】 | 12 - Non Combustible Liquids |
WGK | WGK 2 |