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Anti-c-Fos (Ab-1) Mouse mAb (2G9C3)
产品性质
| Quality Level【质量水平】 | 100 |
| biological source【生物来源】 | mouse |
| antibody form【抗体形式】 | purified antibody |
| antibody product type | primary antibodies |
| clone【克隆】 | 2G9C3, monoclonal |
| form【形式】 | lyophilized |
| does not contain【不包含】 | preservative |
| species reactivity | rat, mouse, human |
| manufacturer/tradename | Calbiochem® |
| storage condition【储存条件】 | OK to freeze |
| isotype【同位素/亚型】 | IgG2a |
| shipped in【运输】 | ambient |
| storage temp.【储存温度】 | 2-8℃ |
| packaging【包装】 | 200 μg in Glass bottle |
基本信息
| General description【一般描述】 | Recognizes both the ~55 kDa c-Fos and ~62 kDa v-Fos proteins in HeLa cells. Does not cross-react with the ~39 kDa Jun protein. This Anti-c-Fos (Ab-1) Mouse mAb (2G9C3) is validated for use in Immunoblotting, Immunocytochemistry, Paraffin Sections for the detection of c-Fos (Ab-1). Purified mouse monoclonal antibody generated by immunizing BALB/c mice with the specified immunogen and fusing splenocytes with AG.8653X mouse myeloma cells. Recognizes the ~55 kDa c-fos and the ~62 kDa v-fos proteins. |
| Immunogen【免疫原】 | a synthetic peptide (KVEQLSPEEEEKRRIRRERNKMAAA) corresponding to amino acids 128-152 of human c-Fos Human |
| Application【应用】 | Immunoblotting (2.5-5 µg/ml) Immunocytochemistry (2.5-5 µg/ml) Paraffin Sections (not recommended) |
| Warning【警告】 | Toxicity: Standard Handling (A) |
| Physical form【外形】 | Lyophilized from a volatile buffer, 200 µg BSA. |
| Reconstitution【重悬】 | Reconstitute in sterile PBS, PH 7.4, or sterile Tris-saline (20 mM Tris containing 150 mM NaCl), pH 7.4, to a final concentration of 200 µg/ml. To achieve complete solubilization, reconstitute at (4°C) with 0.1% sodium azide or aliqout and freeze (-20°C) for long-term storage. Note: sodium azide is cytotoxic. |
| Analysis Note【分析说明】 | Positive Control HeLa cells |
| Other Notes【其他说明】 | Jensen, D.E., et al. 1991. Oncogene6, 1219. Reiss, M., et al. 1990. Cancer Res.50, 6641. RouscherIII, F.J., et al. 1988. Science240, 1010. Sassone-Corsi, P., et al. 1988. Cell54, 553. Giardina, S.L., et al. 1987. Anal Biochem. 161, 109. Verma, I.M., et al. 1987. Cancer Res.49, 29. Verma, I.M., et al. 1987. Cell51, 513. Muller, R. 1986. Viochem. Biophys. Acta.823, 207. Verma, P. 1986. Trends Genet.2, 93. Greenberg, M., et al. 1984. Nature (London)311, 433. Recognizes both cellular and viral forms of fos. Does not react with the ~39 kDa jun protein. Antibody should be titrated for optimal results in individual systems. |
| Legal Information【法律信息】 | CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany |
安全信息
| Storage Class Code【储存分类代码】 | 11 - Combustible Solids |
| WGK | WGK 3 |
