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Anti-Cathepsin B (Ab-1) Mouse mAb (CA10)
产品性质
| Quality Level【质量水平】 | 100 |
| biological source【生物来源】 | mouse |
| antibody form【抗体形式】 | purified antibody |
| antibody product type | primary antibodies |
| clone【克隆】 | CA10, monoclonal |
| form【形式】 | lyophilized |
| does not contain【不包含】 | preservative |
| species reactivity | human, bovine, porcine |
| should not react with | rat |
| manufacturer/tradename | Calbiochem® |
| storage condition【储存条件】 | OK to freeze |
| isotype【同位素/亚型】 | IgG2a |
| shipped in【运输】 | ambient |
| storage temp.【储存温度】 | 2-8℃ |
| packaging【包装】 | 100 μg in Glass bottle |
基本信息
| General description【一般描述】 | Purified mouse monoclonal antibody generated by immunizing BALB/c mice with the specified immunogen and fusing splenocytes with SP2/O-NS1 plasmacytoma cells. Recognizes the ~43 kDa latent and the ~31 kDa active forms of cathepsin B. Anti-Cathepsin B (Ab-1), mouse monoclonal, clone CA10, recognizes the ~43 kDa latent and ~31 kDa active forms of cathepsin B in HepG2 cells. It is validated for WB, IF, and IP. Recognizes the ~43 kDa latent and the ~31 kDa active forms of cathepsin B in HepG2 cells. |
| Immunogen【免疫原】 | purified human liver cathepsin B Human Epitope: within the heavy chain of mature cathepsin B |
| Application【应用】 | Immunoblotting (250 ng/ml) Immunoprecipitation (1 µg/ml) Immunofluorescence (see comments) |
| Warning【警告】 | Toxicity: Standard Handling (A) |
| Physical form【外形】 | Lyophilized from 20 mM ammonium bicabonate buffer, 100 µg BSA (100 µg size) or 20 µg BSA (20 µg size). |
| Reconstitution【重悬】 | Resuspend the lyophilized antibody with 1 ml sterile PBS. Lyophilized antibodies should be resuspended at 4°C with occasional gentle mixing for at least 2 h. Store at 4°C until reconstituted, then store in aliquots at -20°C or at 4°C with 0.1% azide. Freezing of aliquots is best for long-term storage of reconstituted product. Avoid freeze/thaw cycles. |
| Analysis Note【分析说明】 | Positive Control Hep G2 cells |
| Other Notes【其他说明】 | Sloane, B.F. 1990. in Seminars in Cancer Biology, ed. M.M. Gottesman. Vol. 1:137-152. Barret, A.J. 1987. Trends Biochem. Sci.12, 193. Chan, S.J., et al. 1986. Proc. Natl. Acad. Sci. USA83, 7721. Barrett, A.J. and Kirschke, H. 1981. Meth. Enzymol.80, 535. This antibody recognizes an epitope on the heavy chain of mature cathepsin B and therefore detects both mature and pro-cathepsin B. Also reported to work for immunofluorescence. Antibody should be titrated for optimal results in individual systems. |
| Legal Information【法律信息】 | CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany |
安全信息
| Storage Class Code【储存分类代码】 | 11 - Combustible Solids |
| WGK | WGK 1 |
