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Anti-EGFR (Ab-3) Mouse mAb (225)
产品别名
Anti-EGFR (Ab-3) Mouse mAb (225)
Anti-EGF-R
产品性质
| Quality Level【质量水平】 | 100 |
| biological source【生物来源】 | mouse |
| antibody form【抗体形式】 | purified antibody |
| antibody product type | primary antibodies |
| clone【克隆】 | 225, monoclonal |
| form【形式】 | lyophilized |
| does not contain【不包含】 | preservative |
| species reactivity | human |
| should not react with | mouse, rat |
| manufacturer/tradename | Calbiochem® |
| storage condition【储存条件】 | OK to freeze |
| isotype【同位素/亚型】 | IgG1 |
| shipped in【运输】 | ambient |
| storage temp.【储存温度】 | 2-8℃ |
| packaging【包装】 | 100 μg in Plastic ampoule |
基本信息
| General description【一般描述】 | Recognizes the ~170 kDa epidermal growth factor receptor protein in A431 cells. Anti-EGFR (Ab-3), mouse monoclonal, clone 225 recognizes the ~170 kDa epidermal growth factor receptor protein. It is validated for use in immunoprecipitation and neutralization. Purified mouse monoclonal antibody generated by immunizing BALB/c mice with the specified immunogen and fusing splenocytes with NS-1 mouse myeloma cells (see application references). Recognizes the ~170 kDa epidermal growth factor receptor protein. |
| Immunogen【免疫原】 | partially purified, human EGFR from A431 cells Human |
| Application【应用】 | Immunoprecipitation (1-2 µg per sample) Neutralization Studies (2 µg/ml, see comments and application references) |
| Warning【警告】 | Toxicity: Standard Handling (A) |
| Physical form【外形】 | Lyophilized from 20 mM ammonium bicarbonate solution, 100 µg BSA. |
| Reconstitution【重悬】 | Reconstitute the lyophilized antibody with sterile PBS, pH 7.4, or sterile 20 mM Tris-saline (20 mM Tris containing 0.15 M NaCl), pH 7.4, to yield a final concentration of 100 µg/ml. Lyophilized antibodies should be resuspended at 4°C with occasional gentle mixing for at least 2 h. Following reconstitution, aliquot and freeze (-20°C) for long-term storage or refrigerate at 4°C with 0.1% azide for short-term storage. Avoid freeze/thaw cycles of solutions. |
| Analysis Note【分析说明】 | Positive Control A431 cells Negative Control HL-60 cells |
| Other Notes【其他说明】 | Hunter T. 1984. Nature311, 414. Reynolds, F.H., et. al 1981. Nature292, 259. Ushiro, H. and Cohen, S. 1980. J. Biol. Chem.255, 8363. This antibody has been shown to inhibit human tumor xenographs in nude mice. It inhibits EGF binding to its receptor and is an antagonist of in vivo EGF-stimulated tyrosine kinase activity. It also inhibits A431 tumor formation in nude mice. Antibody should be titrated for optimal results in individual systems. |
| Legal Information【法律信息】 | CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany |
安全信息
| Storage Class Code【储存分类代码】 | 12 - Non Combustible Liquids |
| WGK | nwg |
