Anti-EGFR (Ab-5) Mouse mAb (EGFR.1)

100

mouse

purified antibody

primary antibodies

EGFR.1, monoclonal

lyophilized

preservative

human

rat, mouse

Calbiochem^®

OK to freeze

IgG2b

ambient

2-8℃

100 μg in Plastic ampoule

Purified mouse monoclonal antibody generated by immunizing mice with the specified immunogen and fusing splenocytes with NS-1 mouse myeloma cells. Recognizes the ~170 kDa EGF receptor protein.
Anti-EGFR (Ab-5), mouse monoclonal, clone EGFR.1, recognizes the ~170 kDa EGF receptor protein in A431 cells. It is validated for use in FC, IF, IP, and neutralization studies.
Recognizes the ~170 kDa EGF receptor protein in A431 cells.

partially purified, human EGFR from A431 cells
Human

Flow Cytometry (1-5 g/ml)
Immunofluorescence (1-5 g/ml)
Immunoprecipitation (1-2 g/sample)
Neutralization Studies (1-2 g/ml; see application references)

Toxicity: Standard Handling (A)

Lyophilized from a volatile buffer, 100 µg BSA.

We recommend resuspending the lyophilized antibody with sterile PBS, pH 7.4, or sterile 20 mM Tris-saline (20 mM Tris containing 0.15 M NaCl), pH 7.4, to yield a final concentration of 100 µg/ml; product will be more stable if 0.1% sodium azide is included (do not add azide if antibody is to be used with viable cells). Lyophilized antibodies should be resuspended at 4°C with occasional gentle mixing for at least 2 h.

Negative Control
HL-60 cells
Positive Control
A-431 cells

Stains A431, HeLa-5 cells and other human cells but not to rodent cells. It inhibits EGF binding to its receptor and is an antagonist of in vivo EGF-stimulated tyrosine kinase activity. This antibody does not cross-react to c-neu/erb B-2. Antibody should be titrated for optimal results in individual systems.
Hunter T., 1984. Nature311, 414.
Reynolds, F.H., Jr., et al. 1981. Nature292, 259.
Ushiro, H. and Cohen, S. 1980. J. Biol. Chem.255, 8363.

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11 - Combustible Solids

WGK 3