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Anti-Opioid μ Receptor (384-398) Rabbit pAb
产品性质
| biological source【生物来源】 | rabbit |
| Quality Level【质量水平】 | 100 |
| antibody form【抗体形式】 | serum |
| antibody product type | primary antibodies |
| clone【克隆】 | polyclonal |
| form【形式】 | lyophilized |
| does not contain【不包含】 | preservative |
| species reactivity | rat |
| manufacturer/tradename | Calbiochem® |
| storage condition【储存条件】 | OK to freeze |
| isotype【同位素/亚型】 | IgG |
| shipped in【运输】 | ambient |
| storage temp.【储存温度】 | −20℃ |
| packaging【包装】 | 100 μL in Plastic ampoule |
基本信息
| General description【一般描述】 | Recognizes the ~44-45 kDa opiod μ receptor in caudate putamen and the dorsal horn of the spinal cord. This Anti-Opioid µ Receptor (384-398) Rabbit pAb is validated for use in Frozen Sections, Immunoblotting, IF, IP for the detection of Opioid µ Receptor (384-398). Rabbit polyclonal antibody supplied as lyophilized, undiluted serum. Recognizes the ~44-45 kDa opioid µ receptor protein. |
| Immunogen【免疫原】 | Rat a synthetic peptide corresponding to amino acids 384-398 of rat opioid µ receptor |
| Application【应用】 | Frozen Sections (1:500-1:1000, Cy3 technique; 1:3000-1:6000, HRP) Immunoblotting (1:2000-1:2500; see application references) Immunofluorescence (1:100-1:200) Immunoprecipitation (see comments) |
| Warning【警告】 | Toxicity: Highly Toxic (H) |
| Physical form【外形】 | Undiluted serum. |
| Reconstitution【重悬】 | Reconstitute the lyophilized antibody with 100 µl sterile distilled H₂O. Resulting reconstituted solution contains ≤0.1% sodium azide. Be careful to reconstitute the entire contents of the vial; during shipment and handling portions of the lyophilized pellet may have become dislodged and may not be in the bottom of the vial. Following reconstitution, aliquot and freeze (-20°C). |
| Analysis Note【分析说明】 | Positive Control Rat caudate putamen or spinal cord (dorsal horn) |
| Other Notes【其他说明】 | The specificity was determined by immunolabeling of transfected cells, immunoblotting analysis, and immunoisolation studies. Antibody specificity was examined in the rat caudate putamen and dorsal horn of the spinal cord. Staining is completely eliminated by pre-treatment of antibody with immunogen peptide at a concentration of 10 µg/ml. This antibody has also been reported to work for immunopercipitation. Antibody should be titrated for optimal results in individual systems. Zaki, P.A., et al. 1996. Annu. Rev. Pharmacol. Toxicol.36, 379. Arvidsson, U., et al. 1995. J. Neurosci.15, 3328. Kieffer, B.L. 1995. Cell. Mol. Neurobiol.15, 615-635. Childers, S.R. 1991. Life Sci.48, 1991. |
| Legal Information【法律信息】 | CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany |
安全信息
| Storage Class Code【储存分类代码】 | 10 - Combustible liquids |
| WGK | WGK 1 |
