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Anti-Calbindin D-28K (Ab-1) Rabbit pAb
产品性质
| biological source【生物来源】 | rabbit |
| Quality Level【质量水平】 | 100 |
| antibody form【抗体形式】 | serum |
| antibody product type | primary antibodies |
| clone【克隆】 | polyclonal |
| form【形式】 | lyophilized |
| contains【包含】 | ≤0.1% sodium azide as preservative |
| species reactivity | rat, human, baboon, monkey |
| manufacturer/tradename | Calbiochem® |
| storage condition【储存条件】 | OK to freeze avoid repeated freeze/thaw cycles |
| isotype【同位素/亚型】 | IgG |
| shipped in【运输】 | ambient |
| storage temp.【储存温度】 | −20℃ |
| packaging【包装】 | 100 μL in Plastic ampoule |
基本信息
| General description【一般描述】 | Recognizes the Calbindin D-28K protein in rat hippocampus tissue. Anti-Calbindin D-28K (Ab-1), rabbit polyclonal, reecognizes the Calbindin D-28K protein in rat hippocampus. It is validated for use in frozen sections and for T cell co-stimulation. Rabbit polyclonal antibody supplied as lyophilized undiluted serum. Recognizes the calbindin D-28K protein. |
| Immunogen【免疫原】 | Bovine purified bovine cerebellum calbindin D-28K protein |
| Application【应用】 | Frozen Sections (whole mount/vibrotome sections, 1:1000, fluorescence) Vibrotome Sections (1:5000-1:10,000 for biotin-streptavidin/peroxidase detection, see application references) |
| Warning【警告】 | Toxicity: Standard Handling (A) |
| Physical form【外形】 | Undiluted serum. |
| Reconstitution【重悬】 | Reconstitute the lyophilized antibody with 100 µl sterile distilled H₂O. Resulting reconstituted solution will contain ≤0.1% sodium azide. Be careful to reconstitute the entire contents of the vial; during shipment and handling portions of the lyophilized pellet may have become dislodged and may not be in the bottom of the vial. Dilute with sterile PBS or Tris buffer at dilutions no higher than 1:10. Following reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 6 months at -20°C. Avoid freeze/thaw cycles of solutions. |
| Analysis Note【分析说明】 | Positive Control Rat striatum, hippocampus, or cortex |
| Other Notes【其他说明】 | Antibody specificity was examined in the rat striatum, cortex, and hippocampus. Detection of primary antibody with biotin-streptavidin/peroxidase reagents according to manufacturers directions. Antibody should be titrated for optimal results in individual systems. Conde, F., et al. 1994. J. Comp. Neurol.341, 95. Heizmann, C.W. 1993. Acta Neurobiol. Exp.53, 15. Baimbridge, K.G., et al. 1992. Trends Neurosci.15, 303. Heizmann, C.W. and Hunziker, W. 1992. Trends Biochem. Sci.16, 98. |
| Legal Information【法律信息】 | CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany |
安全信息
| Storage Class Code【储存分类代码】 | 10 - Combustible liquids |
| WGK | WGK 1 |
