ATM, active

32160405

NA.32

ATM is a serine/threonine protein kinase which activates checkpoint signaling upon double strand breaks (DSBs), apoptosis and genotoxic stresses such as ionizing ultraviolet A light (UVA), thereby acting as a DNA damage sensor. Recognizes the substrate consensus sequence [ST]-Q. Phosphorylates ′Ser-139′ of histone variant H2AX/H2AFX at double strand breaks (DSBs), thereby regulating DNA damage response mechanism. Also plays a role in pre-B cell allelic exclusion, a process leading to expression of a single immunoglobulin heavy chain allele to enforce clonality and monospecific recognition by the B-cell antigen receptor (BCR) expressed on individual B-lymphocytes. After the introduction of DNA breaks by the RAG complex on one immunoglobulin allele, acts by mediating a repositioning of the second allele to pericentromeric heterochromatin, preventing accessibility to the RAG complex and recombination of the second allele. Also involved in signal transduction and cell cycle control. May function as a tumor suppressor. Necessary for activation of ABL1 and SAPK. Phosphorylates DYRK2, CHEK2, p53/TP53, FANCD2, NFKBIA, BRCA1, CTIP, nibrin (NBN), TERF1, RAD9 and DCLRE1C. May play a role in vesicle and/or protein transport. Could play a role in T-cell development, gonad and neurological function. Plays a role in replication-dependent histone mRNA degradation. Binds DNA ends. Phosphorylation of DYRK2 in nucleus in response to genotoxic stress prevents its MDM2-mediated ubiquitination and subsequent proteasome degradation.
Product Source: Recombinant, human FLAG-tagged ATM full length, expressed in a mammalian cell line.

Routinely evaluated by phosphorylation of recombinant human p53, full length (c-Myc, 6His tagged) as the substrate (Millipore cat # 23-034)

Protein in frozen storage buffer

For Specific Activity data, refer to the Certificate of Analysis for individual lots of this enzyme.

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

100

human

M_w 352 kDa

Upstate^®

activity assay: suitable (kinase)

NM_000051

Q13315

human ... ATM(472)

Also available in 250μg size as catalog # 14-933M and in bulk as catalog # 14-933-K.

6 months at -70°C

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