PI3 Kinase (p110α(E545K)/p85α), 10 μg

32160405

NA.32

Complex of N-terminal 6His-tagged, recombinant, full-length human p110α containing the mutation E545K and untagged, recombinant, full-length human p85α.
The E545K substitution is a somatic mutation in p110α that has been associated with tumours of the colon and brain. Combined in vitro and in vivo studies have shown that this mutation confers higher lipid kinase activity than wild type, and is able to induce oncogenic transformation.

Ni²⁺/NTA-agarose

Store at -70°C from date of shipment. For maximum recovery of product, centrifuge original vial prior to removing the cap.

Rapidly thaw the vial under cold water and immediately place on ice. Aliquot unused material into pre-chilled microcentrifuge tubes and immediately snap-freeze the vials in liquid nitrogen prior to re-storage at -70°C.

100

human

M_w 125.3 kDa (p110α; E545K)
M_w 83.7 kDa (p85α)

Upstate^®

activity assay: suitable (kinase)

NM_006218.2

P42336

human ... PIK3CA(5290)

Protein Target: PI3Kα/p85α
Target Sub-Family: PI3K/PI4K

Also available in 250 μg size --call for pricing and availability and reference catalog number 14-783M when ordering the 250 μg size.

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.