SCF-Fbw7 Protein Complex, Active, 10 μg

32160405

NA.41

The SCF (Skp1-Cul1-F-box protein) complexes represent the largest family of ubiquitin-protein ligases and mediate the ubiquitination of a broad spectrum of regulatory and signalling proteins in diverse cellular pathways. The SCF consists of three invariant components, Skp1, Cul1, and Rbx1 and an interchangeable subunit, an F-box protein which is responsible for recruiting specific substrates to be ubiquitinated by the SCF. SCF^Fbw7 mediates the ubiquitination and subsequent proteasomal degradation of target proteins involved in cell division, growth and differentiation, including Cyclin E1, c-Myc, Notch and c-Jun. Mutations have been found in the Fbw7 subunit of SCF^Fbw7 in various types of human cancers, therefore Fbw7 is considered to be a potent tumour suppressor.
N-terminal c-Myc, 6His-tagged, recombinant human Smad7 full length, expressed by baculovirus in Sf21 insect cells.

Ubiquitination Cascade Comoponent: E3

Purified using glutathione sepharose.

For Specific Activity data, refer to the Certificate of Analysis for individual lots of this enzyme.

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

100

human

Upstate^®

activity assay: suitable

NM_003592
NM_014248
NM_033632
NM_170679

human ... Cul1(8454), Fbw7(55294), Rbx1(9978), Skp1(6500)

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