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UBE1 Protein, active, 10 μg
| 产品编号： | 4094646 |
| 规格： | Active, full length, recombinant human UBE1. N-terminal 6His-tagged. For use in Enzyme Assays. Functions as an E1 enzyme in ubiquitination assays. |
| 包装规格： | 10 μG，250 μG |
| 产品类别： | 进口试剂 |
| 品牌： | Sigma-Aldrich |
| 优惠价： | 立即咨询 |
产品别名
UBE1 Protein, active, 10 μg
Ubiquitin activating enzyme E1, Ubiquitin-like modifier-activating enzyme 1, UBA1, A1S9 Protein, UBE1
基本信息
| eCl@ss | 32160405 |
| NACRES | NA.41 |
| General description【一般描述】 | UBE1 catalyzes the first step in ubiquitin conjugation to mark cellular proteins for degradation. It is responsible for the primary ubiquitin-protein isopeptide bond formation and is a critical component for the initiation of in vitro conjugation reactions. UBE1 activates ubiquitin by the ATP dependent adenylation of its C-terminal glycine carboxyl group. This ubiquitin residue is then linked to the sulphydryl side chain moiety of a cysteine residue within UBE1 by forming a high energy thiol ester bond, liberating free AMP. There are two active sites within the UBE1 molecule allowing it to accommodate two ubiquitin molecules at one time, with a new ubiquitin forming an adenylate intermediate as the previous one is transferred to the thiol site. The activated ubiquitin is then transferred to the lysine of target proteins via the E2/E3 conjugation cascade. N-terminal GST-tagged, recombinant human XIAP full length, expressed by baculovirus in Sf21 insect cells |
| Application【应用】 | Ubiquitination Cascade Comoponent: E1 |
| Physical form【外形】 | Purified using immobilized metal affinity chromatography. |
| Other Notes【其他说明】 | For Specific Activity data, refer to the Certificate of Analysis for individual lots of this enzyme. |
| Legal Information【法律信息】 | UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany |
产品性质
| Quality Level【质量水平】 | 100 |
| biological source【生物来源】 | human |
| manufacturer/tradename | Upstate® |
| technique(s) | activity assay: suitable |
| NCBI accession no.【NCBI登记号】 | NM_003334 |
产品说明
| Disclaimer【免责声明】 | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
