Anti-CD8a Antibody, clone YTS 169.4

32160702

T-cell surface glycoprotein CD8 alpha chain (UniProt: P01731; also known as T-cell surface glycoprotein Lyt-2, CD8a) is encoded by the Cd8a (also known as Lyt-2, Lyt2) gene (Gene ID: 12525) in murine species. CD8 is a single-pass type I membrane protein with one Ig-like V-type (immunoglobulin-like) domain. CD8a is produced with a 27 amino acid signal peptide that is subsequently cleaved. CD8a can form a homodimer (CD8 alpha-alpha), but is more commonly expressed as a heterodimer with a second chain known as CD8b or CD8 beta. CD8 acts as a co-receptor for antigen recognition and subsequent T cell activation that is initiated upon binding of the T cell receptor (TCR) to antigen-bearing MHC Class I molecules. The cytoplasmic domains of CD8 provide binding sites for the tyrosine kinase lck, facilitating intracellular signaling events that lead to T cell activation, development, and cytotoxic effector functions. CD8+ cytotoxic T cells (CTLs) play an important role in inducing cell death of tumor cells, as well as cells infected by virus, bacteria or parasites.

Clone YTS 169.4 detects T-cell surface glycoprotein CD8 alpha chain (CD8a) in mouse thymocytes.

Thymocytes from CBA mouse.

Immunodepletion Analysis: A representative lot detected CD8a in Immunodepletion applications (Cobbold, S.P., et. al. (1984). Nature. 312(5994):548-51; Noort, W.A., et. al. (1996). Transpl Immunol. 4(3):198-202; Brown, C.R., et. al. (1990). J Immunol. 145(10):3438-41).

Flow Cytometry Analysis: A representative lot detected CD8a in Flow Cytometry applications (Noort, W.A., et. al. (1996). Transpl Immunol. 4(3):198-202).

Immunohistochemistry Analysis: A representative lot detected CD8a in Immunohistochemistry applications (Wright, J.R. Jr, et. al. (1997). Ann Transplant. 2(3):12-6).

Anti-CD8a, clone YTS 169.4, Cat. No. MABF1983 is a rat monoclonal antibody that detects T-cell surface glycoprotein CD8 alpha chain and has been tested for use in Flow Cytometry, Immunohistochemistry, and Immunodepletion.
Research Category
Inflammation & Immunology

Evaluated by Flow Cytometry in mouse thymocytes.

Flow Cytometry Analysis: 1 µg of this antibody detected CD8a in mouse thymocytes.

Protein G purified
Format: Purified
Purified rat monoclonal antibody IgG2b in PBS without preservatives.

Concentration: Please refer to lot specific datasheet.

100

rat

purified immunoglobulin

primary antibodies

YTS 169.4, monoclonal

mouse

antibody small pack of 25 μg

flow cytometry: suitable
immunodepletion: suitable
immunohistochemistry: suitable

IgG2bκ

NP_001074579

P01731

27.5 kDa calculated.

Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

12 - Non Combustible Liquids

WGK 2