Milli-Mark Anti-CD3 -PE Antibody, clone UCHT1

32160702

NA.44

Human TCR/CD3 is a complex structure on the lymphocyte surface. It consists of the TCR alpha beta or TCR gamma delta heterodimer and the associated CD3 complex. The CD3 complex is composed of six polypeptides with usually four different transmembrane CD3 chains, gamma, delta, epsilon. and zeta. Three different dimers, gamma epsilon, delta epsilon, and zeta zeta, constitute the CD3 complex. The MW of CD3epsilon is 20 000 (Campana, 1987). CD3 is first detectable in early thymocytes and its appearance probably represents one of the earliest signs of commitment to the T cell lineage. In cortical thymocytes, during early stages of maturation, the CD3 antigen is predominantly present in the cell cytoplasm. In medullary thymocyles, the CD3 antigen is predominantly detected on the cell surface (Swerdlow, 1988; Beverley, 1981). The majority of T cell neoplasms also expresses CD3, but it is absent from non-T-cell lymphoid malignancies (McMichael, 1987). Consistent with the pattern of synthesis of the antigen in normal thymocytes, the earliest site detectable within neoplastic cells is the cell cytoplasm (Swerdlow, 1988).

Antibody recognizes Human CD3 antigen.

Human infant thymocytes and lymphocytes from a patient with Sézary disease.

Research Sub Category
Immunoglobulins & Immunology
Milli-Mark Anti-CD3 -PE Antibody, clone UCHT1 is an antibody against CD3 for use in FC.
Research Category
Inflammation & Immunology

Evaluated by flow cytometry using Human Lymphocytes.

Protein A purified
Purified Mouse monoclonal IgG1 conjugated to PE in 0.05M Tris-HCl buffer with 1% BSA, Sodium Azide at 0.097% at pH 7.2

Control
Human Lymphocytes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

MILLI-MARK is a registered trademark of Merck KGaA, Darmstadt, Germany

100

mouse

PE

purified immunoglobulin

primary antibodies

UCHT1, monoclonal

human

Milli-Mark^®

flow cytometry: suitable

IgG1κ

NP_000724

P07766

wet ice

20 kDa (Campana, 1987)

Maintain refrigerated at 2-8 °C protected from light in undiluted aliquots for up to 6 months from date of receipt.

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

10 - Combustible liquids

WGK 2