Anti-CREB-1 Antibody

32160702

NA.41

Cyclic AMP-responsive element-binding protein (CREB) is a beta ZIP transcription factor that activates target genes through cAMP response elements. CREB is able to mediate signals from numerous physiological stimuli, resulting in regulation of a broad array of cellular responses. While CREB is expressed in numerous tissues, it plays a large regulatory role in the nervous system. CREB is believed to play a key role in promoting neuronal survival, precursor proliferation, neurite outgrowth and neuronal differentiation in certain neuronal populations. Additionally, CREB signaling is involved in learning and memory in several organisms. CREB is able to selectively activate numerous downstream genes through interactions with different dimerization partners. CREB is activated by phosphorylation at Ser133 by various signaling pathways including Erk, Ca2+ and stress signaling. Some of the kinases involved in phosphorylating CREB at Ser133 are p90RSK, MSK, CaMKIV, and MAPKAPK-2.

This antibody recognizes the N-terminus of CREB-1.

KLH-conjugated linear peptide corresponding to the N-terminus of human CREB-1.
Epitope: N-terminus

Immunocytochemistry Analysis: A 1:1,000 dilution from a representative lot detected CREB-1 in PC-12 cells.

Chromatin Immunoprecipitation (ChIP) Analysis: A representative lot detected CREB-1 in HEK293T cells.
Research Sub Category
Transcription Factors
Research Category
Epigenetics & Nuclear Function
Detect CREB using this rabbit polyclonal antibody, Anti-CREB-1 Antibody validated for use in western blotting & ICC.

Evaluated by Western Blotting in A431 cell lysate.

Western Blotting Analysis: 1 µg/mL of this antibody detected CREB-1 in 10 µg of A431 cell lysate.

Replaces: 04-218

Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Affinity purified

Control
A431 cell lysate

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

rabbit

100

affinity isolated antibody

primary antibodies

polyclonal

affinity chromatography

human, rat

equine (based on 100% sequence homology), porcine (based on 100% sequence homology)

ChIP: suitable
immunocytochemistry: suitable
western blot: suitable

NP_004370

P16220

wet ice

human ... CREB1(1385)

~51 kDa observed. A doublet has been observed at 48 kDa in some cell lysates (Rutberg, S. E., et al. (1999). Oncogene. 18(8):1569-1579.). Uncharacterized bands may be observed in some cell lysates.

Stable for 1 year at 2-8°C from date of receipt.

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

12 - Non Combustible Liquids

WGK 1