Anti-Integrin β1 Antibody, clone P4G11, azide free

32160702

NA.41

Human integrin beta1. The epitope recognized by MAB1951Z is Ca2+ dependent.

Human IL-1beta activated endothelial cells

Research Category
Cell Structure
Immunohistochemistry: for use on acetone or mild paraformaldehyde fixed tissue (2-2.5% PFA, short time) frozen and 4% PFA paraffin embedded {Qin et al 2003 1:500, citrate HEIR treated}

Ca++ required for binding. Ca++ must be used in blocking, washing, and antibody incubation buffers.

Traditional formalin fixation is not recommended.

Immunocytochemistry: 2 ug/mL (for use on numerous cell lines as long as Ca2+ is present)

Immunoprecipitation: 5 μg/400 μL lysate (0.5% triton PBS plus Ca++)

FACS: 2-5μg per 10e6 cells, in the presence of Ca++.

Stimulation of cells: 2-10 μg/ml per 10e6 cells. Binding buffer must contain 1mM Ca++.

ELISA

Optimal working dilutions must be determined by end user.
Research Sub Category
Integrins
This Anti-Integrin β1 Antibody, clone P4G11, azide free is validated for use in ELISA, FC, IP, FUNC, FUNC, IC, IH for the detection of Integrin β1.

Format: Purified
Sterile-filtered protein A purified immunoglobulin in 0.01M PBS, pH 7.1, containing no preservatives.

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

100

mouse

purified immunoglobulin

primary antibodies

P4G11, monoclonal

rabbit, human, monkey

Chemicon^®

ELISA: suitable
flow cytometry: suitable
immunocytochemistry: suitable
immunohistochemistry: suitable
immunoprecipitation (IP): suitable

IgG1

NM_002211.2
NM_033666.1
NM_033667.1
NM_033668.1
NM_033669.1
NM_133376.1

P05556

wet ice

human ... ITGB1(3688)

Maintain between 2 and 8°C for up to 6 months from date of receipt. Avoid freeze thaws.

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

12 - Non Combustible Liquids

WGK 2