Anti-Integrin αVβ6 Antibody, clone E7P6, azide free

32160702

NA.41

Monoclonal antibody MAB2074Z, raised by immunizing Balb/c mice with recombinant secreted human avb6, is useful for flow cytometry and is especially useful for immunocytochemistry. The antibody works well for immunohistochemistry of human tissue, especially under circumstances where upregulation of the receptor is being evaluated. The antibody does not stain formalin-fixed or paraffin-embedded tissues, and may not detect the low-level basal expression of alphavbeta6 in epithelia of healthy adults. This antibody does not recognize the alphaV subunit and does not react with alphaV integrins other than alphavbeta6. Because the antibody is not effective in western blot, it is unknown whether the epitope recognized by the antibody localizes to the alphavbeta6 heterodimer or to the beta6 subunit only.

Recombinant secreted human alphavbeta6.

Research Category
Cell Structure
Research Sub Category
Integrins
Immunohistochemistry (acetone fixation): Effective for human tissue, especially in circumstances where up-regulation of the receptor is being evaluated. The antibody does not stain formalin-fixed or paraffin-embedded tissues, and may not detect the low-level basal expression of alpavbeta6 in epithelia of healthy adults.

Immunocytochemistry: Effective for F6, HT-29, H441 cell lines (others not tested).

Flow cytometry: Effective for detection of human and monkey alphaVbeta6

Not effective for immunoprecipitation or western blot.

Optimal working dilutions must be determined by end user.
This Anti-Integrin αVβ6 Antibody, clone E7P6, azide free is validated for use in FC, IC, IH for the detection of Integrin αVβ6.

Sterile-filtered liquid at 1 mg/mL in PBS, containing no preservatives.
Format: Purified

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

100

mouse

purified immunoglobulin

primary antibodies

E7P6, monoclonal

human

Chemicon^®

flow cytometry: suitable
immunocytochemistry: suitable
immunohistochemistry: suitable

IgG1

not suitable for activity/function inhibition
not suitable for immunohistochemistry (Paraffin)

NM_002210.2

P06756

wet ice

human ... ITGAV(3685)

Maintain at 2-8°C in undiluted aliquots for up to 12 months.

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

12 - Non Combustible Liquids

WGK 2