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Raf-1 RBD Protein, GST, 300 μg

产品编号:4101747
规格:GST fusion-protein, corresponding to the human Ras Binding Domain (RBD, residues 1-149) of Raf-1, expressed in E. coli. with purity 50% at full length molecular weight 42 kDa. For use in Affinity Binding Assays
包装规格:300 μG
产品类别:进口试剂
品牌:Sigma-Aldrich
优惠价:立即咨询
产品价格
产品编号包装单位单价(元)国内现货国外库存询价单
4101747300 μG7760
基本信息
eCl@ss
32160405
NACRES
NA.41
General description【一般描述】
Product Source: Expressed in E. coli
GST fusion-protein, corresponding to the human Ras Binding Domain (RBD, residues 1-149) of Raf-1, expressed in E. coli. with purity >50% at full length molecular weight 42 kDa.
Specifically binds to and precipitates Ras-GTP from cell lysate. Can be bound to glutathione coated plates prior to precipitating Ras-GTP.
Application【应用】
Specifically binds to and precipitates Ras-GTP from cell lysate.Can be bound to glutathione coated plates prior to precipitating Ras-GTP.
Research Sub Category
Obesity

Metabolic Disorders
Research Category
Metabolism
Quality【质量】
Affinity Precipitation of Activated Ras: Raf-1-RBD, GST can be captured on a glutathione coated plate and used to capture GTP activated Ras from HeLa cell lysate. Used 2 μg of Raf-1-RBD, GST per well to capture 25-100 μg of EGF stimulated HeLa cell lysate. Upstate also offers a Ras GTPase ELISA kit (17-424).
Note: Due to spontaneous deactivation of Ras, use lysates that have been stored at -70°C for less than 6 month.
Physical form【外形】
Glutathione-agarose
300 μg of Raf-1 RBD, GST in 150 μL in PBS, pH 7.4, 0.5mM PMSF, and 20% glycerol.
Legal Information【法律信息】
UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany
产品性质
Quality Level【质量水平】
100
biological source【生物来源】
human
Assay【测定】
>50%
mol wt【分子量】
Mw 42 kDa
manufacturer/tradename
Upstate®
technique(s)
affinity binding assay: suitable
NCBI accession no.【NCBI登记号】
NM_002880.2
UniProt accession no.【UniProt登记号】
P04049
shipped in【运输】
dry ice
Gene Information
human ... RAF1(5894)
产品说明
Storage and Stability【储存及稳定性】
1 year at -20°C from date of shipment.
Disclaimer【免责声明】
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
安全信息
Storage Class Code【储存分类代码】
10 - Combustible liquids
WGK
WGK 2
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