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Anti-Heat Shock Protein 70 Antibody, clone W27
产品别名
Anti-Heat Shock Protein 70 Antibody, clone W27
hsp70
基本信息
eCl@ss | 32160702 |
NACRES | NA.41 |
Specificity【特异性】 | Reacts with both the constitutive (HSP73) and inducible (HSP72) forms of HSP70. POSITIVE CONTROL: HeLa cells, BT474 cells or breast carcinoma. |
Immunogen【免疫原】 | HSP70 from HeLa cells. |
Application【应用】 | Immunoblotting: 0.5-1 μg/mL Immunohistochemistry (frozen and formalin/paraffin): 2-4 μg/mL for 2 hours at room temperature. Staining of formalin fixed tissue sections requires boiling the tissue sections in 10mM citrate buffer, pH 6.0 for 10-20 minutes followed by cooling at room temperature for 20 minutes. Immunoprecpitation: 2 μg/mg protein lysate (use Protein G) Flow cytometry Optimal working dilutions must be determined by end user. This Anti-Heat Shock Protein 70 Antibody, clone W27 is validated for use in IP, WB, IH(P) for the detection of Heat Shock Protein 70. |
Physical form【外形】 | Format: Purified |
Analysis Note【分析说明】 | Control POSITIVE CONTROL: BT474, MAD109, RenCa, SK-NEP-1, U937 cells or breast carcinoma. |
Other Notes【其他说明】 | Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration. |
Legal Information【法律信息】 | CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany |
产品性质
Quality Level【质量水平】 | 100 |
biological source【生物来源】 | mouse |
antibody form【抗体形式】 | purified antibody |
antibody product type | primary antibodies |
clone【克隆】 | W27, monoclonal |
species reactivity | human, monkey, bovine |
manufacturer/tradename | Chemicon® |
technique(s) | immunohistochemistry: suitable (paraffin) immunoprecipitation (IP): suitable western blot: suitable |
isotype【同位素/亚型】 | IgG2a |
NCBI accession no.【NCBI登记号】 | NM_002154.3 NM_198431.1 |
UniProt accession no.【UniProt登记号】 | P34932 |
shipped in【运输】 | wet ice |
Gene Information | human ... HSPA4(3308) |
产品说明
【免责声明】 | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
安全信息
Storage Class Code【储存分类代码】 | 12 - Non Combustible Liquids |
WGK | WGK 2 |