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Anti-c-Ret Antibody, clone 6F3.1
产品别名
Anti-c-Ret Antibody, clone 6F3.1
Proto-oncogene tyrosine-protein kinase receptor Ret, Cadherin family member 12, Proto-oncogene c-Ret
基本信息
eCl@ss | 32160702 |
NACRES | NA.41 |
General description【一般描述】 | Proto-oncogene tyrosine-protein kinase receptor Ret (EC 2.7.10.1; UniProt P07949; also known as Cadherin family member 12, Cadherin-related family member 16, Hydroxyaryl-protein kinase, Proto-oncogene c-Ret, Rearranged during transfection, Receptor tyrosine kinase, Ret proto-oncogene, RET receptor tyrosine kinase, RET transforming sequence) is encoded by the RET (also known as CDHF12, CDHR16, HSCR1, MEN2A, MEN2B, MTC1, PTC, RET-ELE1, RET51) gene (Gene ID 5979) in human. Ret is a receptor tyrosine kinase that mediates the activation of several signaling pathways including the PLC gamma, Ras, JNK and inositol phosphate pathways. Ret mutations have been shown to be causative in several diseases, including Hirschsprung′s disease (HD), papillary thyroid carcinoma, multiple endocrine neoplasia (MEN) 2A, MEN 2B, and familial medullary thyroid carcinoma. Ret is initially produced with a signal peptide sequence (a.a. 1-28), the removal of which yields the mature Ret (a.a. 20-1114). Ret can be posttranslationally cleaved into the extracellular cell-membrane-anchored RET cadherin 120 kDa fragment (a.a. 29-707) and the soluble RET kinase fragment (a.a. 708-1017). |
Specificity【特异性】 | Expected to react with both the RET51 and the RET9 isoforms, as well as the extracellular cell-membrane anchored RET cadherin 120 kDa fragment (a.a. 29-707). |
Immunogen【免疫原】 | Epitope: Extracellular domain GST-tagged recombinant protein corresponding to the extracellular domain of human c-Ret. |
Application【应用】 | Research Sub Category Apoptosis - Additional Research Category Apoptosis & Cancer This Anti-c-Ret Antibody, clone 6F3.1 is validated for use in Western Blotting for the detection of c-Ret. |
Quality【质量】 | Evaluated by Western Blotting in PC12 cell lysate. Western Blotting Analysis: 1.0 µg/mL of this antibody detected c-Ret in 10 µg of PC12 cell lysate. |
Physical form【外形】 | Purified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide. Format: Purified Protein G Purified |
Other Notes【其他说明】 | Concentration: Please refer to lot specific datasheet. |
产品性质
Quality Level【质量水平】 | 100 |
biological source【生物来源】 | mouse |
antibody form【抗体形式】 | purified immunoglobulin |
antibody product type | primary antibodies |
clone【克隆】 | 6F3.1, monoclonal |
species reactivity | rat |
species reactivity (predicted by homology) | human (based on 100% sequence homology) |
technique(s) | western blot: suitable |
isotype【同位素/亚型】 | IgG1κ |
NCBI accession no.【NCBI登记号】 | NP_066124 |
UniProt accession no.【UniProt登记号】 | P07949 |
shipped in【运输】 | wet ice |
Gene Information | human ... RET(5979) |
产品说明
Target description【目标描述】 | ~150 kDa observed. 124.3 kDa (RET51) and 119.8 kDa (RET9) calculated. Target band(s) may appear larger than the calculated molecular size(s) due to posttranslational glycosylation and/or phosphorylation. Uncharacterized band(s) may appear in some lysates. |
Storage and Stability【储存及稳定性】 | Stable for 1 year at 2-8°C from date of receipt. |
Disclaimer【免责声明】 | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
安全信息
Storage Class Code【储存分类代码】 | 12 - Non Combustible Liquids |
WGK | WGK 1 |