Anti-p40 Antibody

32160702

NA.41

p40, also known as Tumor protein 63, p63, Chronic ulcerative stomatitis protein, CUSP, Keratinocyte transcription factor , KET, Transformation-related protein 63, TP63, Tumor protein p73-like, p73L, p40, or p51, and encoded by the gene TP63/KET/P63/P73H/P73L/TP73L, is a member of the p53 protein family. p40 is a sequence specific DNA binding transcriptional activator or repressor depending upon the target gene and the isoform involved. The isoforms contain a varying set of transactivation and auto-regulating transactivation inhibiting domains thus showing an isoform specificactivity. p40 is involved in Notch signaling and plays a role in p53/TP53 dependent apoptosis and epithelial morphogenesis. Moreover p40, developmentally, is required for proper limb formation. p40 is expressed widely, notably in heart, kidney, placenta, prostate, skeletal muscle, testis and thymus, although the precise isoform varies according to tissue type. Progenitor cell layers of skin, breast, eye and prostate express high levels of DeltaN-type isoforms. Isoform 10 is predominantly expressed in skin squamous cell carcinomas, but not in normal skin tissues. Mutations in p40 lead to a variety of dysplasias and malformations, including Acro-dermato-ungual-lacrimal-tooth syndrome (ADULT syndrome), Ankyloblepharon-ectodermal defects cleft lip/palate disorder (AEC), Ectrodactyly, ectodermal dysplasia, and cleft lip/palate syndrome 3 (EEC3), Split-hand/foot malformation 4 (SHFM4), Limb-mammary syndrome (LMS), Ectodermal dysplasia, Rapp-Hodgkin type (EDRH), Non-syndromic orofacial cleft 8 (OFC8).

Uniprot describes multiple isoforms for this protein and based on the immunogen this antibody should recognize isoforms 2,4,6,8,10 and 12.

KLH-conjugated linear peptide corresponding to the transcription activation domain of human p40.
Epitope: Transcription activation domain

Western Blotting Analysis: 2.0 µg/mL from a representative lot detected p40 in 10 µg of human liver and human skin tissue lysate.
Immunohistochemistry Analysis: A 1:1,000 dilution from a representative lot detected Isocitrate dehydrogenase in squamous carcinoma cells.
Anti-p40 | ABS552 is an antibody against p40 for use in Western Blotting, Immunohistochemistry (Paraffin).
Research Category
Signaling
Research Sub Category
Signaling Neuroscience

Evaluated by Western Blotting in human heart tissue lysate.

Western Blotting Analysis: 2.0 µg/mL of this antibody detected p40 in 10 µg of human heart tissue lysate.

Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Affinity purified

Concentration: Please refer to lot specific datasheet.

300
100

rabbit

affinity isolated antibody

primary antibodies

polyclonal

affinity chromatography

human

mouse (based on 100% sequence homology)

immunohistochemistry: suitable (paraffin)
western blot: suitable

NP_001108454

Q9H3D4

wet ice

~63/56/51/46 kDa observed. Uncharacterized band(s) may appear in some lysates.

Stable for 1 year at 2-8°C from date of receipt.

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

12 - Non Combustible Liquids

WGK 1