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Anti-phospho BAD Antibody (Ser155)
| 产品编号： | 4102560 |
| 规格： | from rabbit, purified by affinity chromatography |
| 包装规格： | 200 μL |
| 产品类别： | 进口试剂 |
| 品牌： | Sigma-Aldrich |
| 优惠价： | 立即咨询 |
产品别名
Anti-phospho BAD Antibody (Ser155)
Bcl2 antagonist of cell death, BAD, Bcl-2-binding component 6, Bcl-2-like protein 8, Bcl2-L-8, Bcl-XL/Bcl-2-associated death promoter
基本信息
| eCl@ss | 32160702 |
| NACRES | NA.41 |
| General description【一般描述】 | Bad is a member of the BCL-2 family. BCL-2 family members are known to be regulators of programmed cell death. This protein positively regulates cell apoptosis by forming heterodimers with BCL-xL and BCL-2, and reversing their death repressor activity. Proapoptotic activity of this protein is regulated through its phosphorylation. Protein kinases AKT and MAP kinase, as well as protein phosphatase calcineurin are found to be involved in the regulation of this protein. Bad is phosphorylated on one or more of Ser-75, Ser-99, Ser-118, Ser-134, and Ser-155 in response to survival stimuli, which blocks its pro-apoptotic activity. Phosphorylation on Ser-99 or Ser-75 promotes heterodimerization with 14-3-3 proteins. This interaction then facilitates the phosphorylation at Ser-118, a site within the BH3 motif, leading to the release of Bcl-X(L) and the promotion of cell survival. Ser-99 is the major site of AKT/PKB phosphorylation, Ser-118 the major site of protein kinase A (CAPK) phosphorylation. |
| Immunogen【免疫原】 | Epitope: BH3 domain KLH-conjugated linear peptide corresponding to the BH3 domain of human phospho BAD (Ser155). |
| Application【应用】 | Research Sub Category Apoptosis - Additional Peptide Inhibition Analysis: A 1:100 dilution from a representative lot blocked phospho BAD (Ser155) in Forskolin treated PC12 cell lysates. Immunoprecipitation Analysis: 5 µg from a representative lot immunoprecipitated phospho BAD (Ser155) in 0.5 mg of Forskolin treated PC12 cell lysate. Anti-phospho BAD (Ser155) is an antibody against phospho BAD (Ser155) for use in WB, PIA, IP. Research Category Apoptosis & Cancer |
| Quality【质量】 | Evaluated by Western Blotting in Forskolin treated PC12 cell lysate. Western Blotting Analysis: A 1:250 dilution of this antibody detected phospho BAD (Ser155) in 10 µg of Forskolin treated PC12 cell lysate. |
| Physical form【外形】 | Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide. Affinity purified |
| Other Notes【其他说明】 | Concentration: Please refer to lot specific datasheet. |
产品性质
| biological source【生物来源】 | rabbit |
| Quality Level【质量水平】 | 100 |
| antibody form【抗体形式】 | affinity isolated antibody |
| antibody product type | primary antibodies |
| clone【克隆】 | polyclonal |
| purified by【纯化方式】 | affinity chromatography |
| species reactivity | human |
| species reactivity (predicted by homology) | canine (based on 100% sequence homology), rat (based on 100% sequence homology), bovine (based on 100% sequence homology), mouse (based on 100% sequence homology) |
| technique(s) | immunoprecipitation (IP): suitable inhibition assay: suitable (peptide) western blot: suitable |
| NCBI accession no.【NCBI登记号】 | NP_031548 |
| UniProt accession no.【UniProt登记号】 | Q92934 |
| shipped in【运输】 | dry ice |
产品说明
| Target description【目标描述】 | ~20 kDa observed. Uncharacterized band(s) may appear in some lysates. |
| Storage and Stability【储存及稳定性】 | Stable for 1 year at 2-8°C from date of receipt. |
| Disclaimer【免责声明】 | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
安全信息
| Storage Class Code【储存分类代码】 | 12 - Non Combustible Liquids |
| WGK | WGK 1 |
