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Anti-phospho-GRK2 (Ser670) Antibody, clone 15G11.1
产品编号: | 4102577 |
规格: | clone 15G11.1, from mouse, unconjugated |
包装规格: | 100 μL |
产品类别: | 进口试剂 |
品牌: | Sigma-Aldrich |
优惠价: | 立即咨询 |
产品别名
Anti-phospho-GRK2 (Ser670) Antibody, clone 15G11.1
Beta-adrenergic receptor kinase 1, Beta-ARK-1, G-protein coupled receptor kinase 2
基本信息
eCl@ss | 32160702 |
NACRES | NA.41 |
General description【一般描述】 | G-protein coupled receptor kinase 2 (Grk2), also known as BARK or BARK1, is a member of the large family of GRK kinases that phosphorylate G-protein coupled receptors (GPCR), resulting in the recruitment of arrestins to the cytoplasmic domains of GPCR’s and subsequent internalization of these receptors by endocytosis. GRK2 is ubiquitously expressed, and modulates diverse cellular processes through association with a number of non-receptor substrates. GRK2 regulates actin dynamics and cell motililty through interaction with radixin, tubilin, and ezrin. In addition, Grk2 may play a role in the signaling of TGFβ by blocking the nuclear translocation of Smad2 and Smad3 proteins. It has also been shown that Grk2 may play a role in the hedgehog signaling pathway during embryonic development, by modulating the signaling of the atypical G-protein coupled receptor Smo. Grk2 is inactivated by phosphorylation of ser670 by ERK. It is also phosphorylated by PKC and Src, depending on the cellular context. |
Specificity【特异性】 | This antibody recognizes GRK2 phosphorylated at Ser670. |
Immunogen【免疫原】 | Epitope: Phosphorylated Ser670 KLH-conjugated linear peptide corresponding to human GRK2 phosphorylated at Ser670. |
Application【应用】 | Research Sub Category Hormones & Receptors Research Category Signaling Peptide Inhibition Assay Analysis: A 1:1,000 dilution from a representative lot blocked phospho-GRK2 in 10 µg of Calyculin A/Okadaic Acid treated and untreated Jurkat cells. Immunoprecipitation Analysis: A representative lot immunoprecipitated phospho-GRK2 in HeLa cell lysate. Anti-phospho-GRK2 (Ser670) Antibody, clone 15G11.1 is an antibody against phospho-GRK2 (Ser670) for use in Western Blotting, Immunoprecipitation, Peptide Inhibition Assay. |
Quality【质量】 | Evaluated by Western Blot in untreated and Calyculin A/Okadaic Acid treated A431 cells. Western Blot Analysis: A 1:1,000 dilution of this antibody detected GRK2 in 10 µg of untreated and Calyculin A/Okadaic Acid treated A431 cells. |
Physical form【外形】 | Purified mouse monoclonal IgG2bκ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide. Protein G Purified |
Analysis Note【分析说明】 | Control Untreated and Calyculin A/Okadaic Acid treated A431 cells |
产品性质
Quality Level【质量水平】 | 100 |
biological source【生物来源】 | mouse |
conjugate【偶联物】 | unconjugated |
antibody form【抗体形式】 | purified immunoglobulin |
antibody product type | primary antibodies |
clone【克隆】 | 15G11.1, monoclonal |
species reactivity | human |
technique(s) | immunoprecipitation (IP): suitable inhibition assay: suitable (peptide) western blot: suitable |
isotype【同位素/亚型】 | IgG2bκ |
NCBI accession no.【NCBI登记号】 | NP_001610 |
UniProt accession no.【UniProt登记号】 | P25098 |
shipped in【运输】 | wet ice |
产品说明
Target description【目标描述】 | ~70 kDa observed |
Storage and Stability【储存及稳定性】 | Stable for 1 year at 2-8°C from date of receipt. |
Disclaimer【免责声明】 | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
安全信息
Storage Class Code【储存分类代码】 | 12 - Non Combustible Liquids |
WGK | WGK 1 |