Anti-Stage-Specific Embryonic Antigen-4 Antibody, clone MC-813-70

32160702

NA.41

The cell-surface markers used routinely to identify undifferentiated human embryonic stem cells (hESCs) were initially characterized as markers for mESCs, mouse embryonic carcinomas (ECs), or human EC cells. Stage-specific embryonic antigen-3 (SSEA-3) and SSEA-4 are widely used as cell-surface markers to define both human and mouse ESCs. SSEA-3 and SSEA-4 are expressed on undifferentiated hESCs but not on undifferentiated mESCs.

This antibody reacts with the Stage-specific embryonic antigen-4 (SSEA-4) that is expressed upon the surface of human tetracarcinoma stem cells (EC), human embryonic germ cells (EG) and human embryonic stem cells (ES). No immunoreactivity is evident with undifferentiated murine EC, ES and EG cells. Expression of SSEA-4 is down regulated following differentiation of human EC cells. In contrast, the differentiation of murine EC and ES cells may be accompanied by an increase in SSEA-4 expression.

Human embryonal carcinoma cell line 2102Ep

Research Sub Category
Pluripotent & Early Differentiation
Research Category
Stem Cell Research
Anti-Stage-Specific Embryonic Antigen-4 Antibody, clone MC-813-70 is an antibody against Stage-Specific Embryonic Antigen-4 for use in FC, ELISA, IF, IH.
Immunohistochemistry: A previous lot of this antibody was used in IH.

Immunofluorescence: A previous lot of this antibody was used in IF.

ELISA: A previous lot of this antibody was used in ELISA.

Flow Cytometry: A starting range of 10-20 µg/mL is suggested.

Optimal working dilutions must be determined by the end user.

Purified moust monoclonal IgG3 liquid in buffer containing 0.02 M PBS, 0.25 M NaCl, pH 7.6 with 0.1% Sodium Azide
Format: Purified
Protein A purified

Control
Pluripotent human embryonic stem (ES) cells

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

100

mouse

purified immunoglobulin

primary antibodies

MC-813-70, monoclonal

human, mouse

Chemicon^®

ELISA: suitable
flow cytometry: suitable
immunofluorescence: suitable
immunohistochemistry: suitable

sample type: human embryonic stem cell(s)
sample type induced pluripotent stem cell(s)
sample type: mouse embryonic stem cell(s)

IgG3

wet ice

Stable for 1 year at from date of receipt.

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

10 - Combustible liquids

WGK 2