Anti-Tumor Necrosis Factor-α Antibody, clone 2C8

32160702

NA.41

Human tumor necrosis factor-a (TNF-a)
No cross-reactivity observed with rat neuronal tissue.

Human recombinant tumor necrosis factor α.

Detect Tumor Necrosis Factor-α using this Anti-Tumor Necrosis Factor-α Antibody, clone 2C8 validated for use in ELISA, IH, NEUT.
Inhibition Assays – Inhibition of biological activity of recombinant and natural TNF-α.
Detection of TNF-α in two-site immunoassay.

Immunohistochemistry detected TNF-α.

TNF-α Neutralization Assay

1. Plate 2 x 10⁴ murine L929B cells into individual wells of 96-well (flat bottomed) microtiter plate.

2. Incubate overnight at 37°C in humidified 5% CO₂ incubator.

3. The next day, prepare serial dilutions of anti-TNF (human) in medium containing 2 μg/mL of actinomycin D, for a final volume of 50 μL

4. Add 50 μL of TNF (10 ng/mL final concentration) to each antibody dilution and incubate for 1 hour at 37°C.

5. Add 100 μL of the antibody/antigen mixture to the pre-seeded L929B cells.

6. Incubate 24 hours at 37°C, 5% CO₂.

7. Score wells for cytotoxicity.

Optimal working dilutions must be determined by the end user.

Replaces: 04-1114

Protein A purified
Format: Purified

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

100

mouse

purified antibody

2C8, monoclonal

human

Chemicon^®

ELISA: suitable
immunohistochemistry: suitable
neutralization: suitable

IgG1

NM_000594.2

P01375

wet ice

human ... TNF(7124)

Maintain at 2-8°C in undiluted aliquots for up to one year.

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

10 - Combustible liquids

WGK 2