您的位置：首页 > 产品中心 > IL-12 human
IL-12 human
| 产品编号： | 4102957 |
| 规格： | recombinant, expressed in CHO cells, ≥98% (SDS-PAGE), ≥98% (HPLC), suitable for cell culture |
| 包装规格： | 10 μG |
| 产品类别： | 进口试剂 |
| 品牌： | Sigma-Aldrich |
| 优惠价： | 立即咨询 |
产品别名
IL-12 human
CTL maturation factor (TCMF),
TSF
Cytotoxic lymphocyte maturation factor (CLMF)
NKSF
产品性质
| biological source【生物来源】 | human |
| recombinant【重组】 | expressed in CHO cells |
| Assay【测定】 | ≥98% (HPLC) ≥98% (SDS-PAGE) |
| form【形式】 | lyophilized |
| potency【效能】 | ≤1.0 ng/mL ED50 |
| mol wt【分子量】 | 40 kDa |
| packaging【包装】 | pkg of 10 μg |
| technique(s) | cell culture | mammalian: suitable |
| impurities【杂质】 | <0.1 EU/μg endotoxin, tested |
| color【颜色】 | white to off-white |
| UniProt accession no.【UniProt登记号】 | P29459 P29460 |
| shipped in【运输】 | wet ice |
| storage temp.【储存温度】 | −20℃ |
| Gene Information | human ... IL12A(3592) |
基本信息
| General description【一般描述】 | IL-12 (interleukin 12) is produced by activated monocytes/macrophage cells, B lymphocytes and connective tissue type mast cells. Recombinant Human IL-12 is a 75kDa heterodimeric glycoprotein consisting of disulfide-linked 35kDa (p35) and 40kDa (p40) subunits (503 total amino acid residues). |
| Biochem/physiol Actions【生化/生理作用】 | IL-12 (interleukin 12) is a proinflammatory cytokine and plays an important role in protection against pathogens. IL-12 is a potent regulator of cell mediated immune responses and it induces IFN (interferon)-γ production by NK (natural killer) and T cells. Among its biological activities IL-12 promotes the growth and activity of activated NK, CD4+ and CD8+ cells and induces the development of IFN-γ producing Th1 (T helper) cells. Polymorphisms in the IL-12 gene are associated with risk of rheumatoid arthritis. It also exhibits anti-tumor activities by enhancing Th1 adaptive immunity and cytotoxic T lymphocyte functions. |
| Sequence【序列】 | p35 Subunit: RNLPVATPDP GMFPCLHHSQ NLLRAVSNML QKARQTLEFY PCTSEEIDHE DITKDKTSTV EACLPLELTK NESCLNSRET SFITNGSCLA SRKTSFMMAL CLSSIYEDLK MYQVEFKTMN AKLLMDPKRQ IFLDQNMLAV IDELMQALNF NSETVPQKSS LEEPDFYKTK IKLCILLHAF RIRAVTIDRV MSYLNAS p40 Subunit: IWELKKDVYV VELDWYPDAP GEMVVLTCDT PEEDGITWTL DQSSEVLGSG KTLTIQVKEF GDAGQYTCHK GGEVLSHSLL LLHKKEDGIW STDILKDQKE PKNKTFLRCE AKNYSGRFTC WWLTTISTDL TFSVKSSRGS SDPQGVTCGA ATLSAERVRG DNKEYEYSVE CQEDSACPAA EESLPIEVMV DAVHKLKYEN YTSSFFIRDI IKPDPPKNLQ LKPLKNSRQV EVSWEYPDTW STPHSYFSLT FCVQVQGKSK REKKDRVFTD KTSATVICRK NASISVRAQD RYYSSSWSEW ASVPCS |
| Physical form【外形】 | Lyophilized from 0.5x PBS, pH 7.2. |
| Reconstitution【重悬】 | Centrifuge the vial prior to opening. Reconstitute in 1x PBS, pH 7.2-7.4 to a concentration of 0.2 mg/ml. Do not vortex. This solution can be stored at 2-8℃ for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20℃ to -80℃. |
安全信息
| Storage Class Code【储存分类代码】 | 13 - Non Combustible Solids |
| WGK | WGK 3 |
