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MIF human
产品编号: | 4103043 |
规格: | recombinant, expressed in insect cells, ≥98% (SDS-PAGE), ≥98% (HPLC) |
包装规格: | 25 μG |
产品类别: | 进口试剂 |
品牌: | Sigma-Aldrich |
优惠价: | 立即咨询 |
产品别名
MIF human
MMIF
GIF
GLIF
基本信息
NACRES | NA.32 |
General description【一般描述】 | Macrophage migration inhibitory factor (MIF) is a small secreted protein that can act as a pleiotropic pro-inflammatory cytokine as well as an enzyme. MIF pro-inflammatory activity can be initiated by signaling through cluster of differentiation 74 and 44 (CD74 and CD44), resulting in the secretion of tumor necrosis-α (TNF-α), interleukins-1, -6, -8, and various matrix metalloproteinases (MMPs). The gene encoding this protein is localized on human chromosome 22q11.23. Insect cell derived recombinant MIF is a 15kDa protein containing 124 amino acid residues, including an N-terminal His-tag. |
Biochem/physiol Actions【生化/生理作用】 | The enzymatic activity of macrophage migration inhibitory factor (MIF) is characterized by its ability to act as a tautomerase, capable of catalyzing the keto to enol isomerization of keto-phenylpyruvate and L-dopachrome. It appears as though MIF catalytic activity is dependent upon a trimeric configuration and a free N-terminal proline residue. |
Physical form【外形】 | Lyophilized from 10mM Sodium Phosphate, pH 7.0. |
Reconstitution【重悬】 | Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/ml. Do not vortex. This solution can be stored at 2-8℃ for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20℃ to -80℃. |
产品性质
biological source【生物来源】 | human |
recombinant【重组】 | expressed in insect cells |
Assay【测定】 | ≥98% (HPLC) ≥98% (SDS-PAGE) |
form【形式】 | lyophilized |
mol wt【分子量】 | 15.0 kDa |
packaging【包装】 | pkg of 25 μg |
impurities【杂质】 | endotoxin, tested |
UniProt accession no.【UniProt登记号】 | P14174 |
shipped in【运输】 | wet ice |
storage temp.【储存温度】 | −20℃ |
Gene Information | human ... MIF(4282) |
安全信息
Storage Class Code【储存分类代码】 | 11 - Combustible Solids |
WGK | WGK 3 |