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TIMP-1 human
| 产品编号： | 4103287 |
| 规格： | recombinant, expressed in E. coli, ≥95% (SDS-PAGE), ≥95% (HPLC), suitable for cell culture |
| 包装规格： | 10 μG |
| 产品类别： | 进口试剂 |
| 品牌： | Sigma-Aldrich |
| 优惠价： | 立即咨询 |
产品别名
TIMP-1 human
Erythroid-potentiating activity
Fibroblast collagenase inhibitor
Tissue inhibitor of metalloproteinase
基本信息
| NACRES | NA.32 |
| General description【一般描述】 | TIMP1 (tissue inhibitor of metalloproteinase 1) is a glycoprotein and belongs to the TIMP family of endogenous MMP (matrix metalloproteinase) inhibitors. Humans contain four TIMPs. All TIMPs contain an N-terminal of 125 residues, a 65-residue C-terminal, and both these domains containing three disulfide bonds. The N-terminal domain inhibits MMPs by folding into a separate unit. Recombinant human TIMP-1 is a 20.6 kDa protein containing 184 amino acid residues. |
| Biochem/physiol Actions【生化/生理作用】 | TIMP1 functions as a poor inhibitor of MT1 (membrane type 1)-MMP, MT3-MMP, MT5-MMP and MMP-19. It inhibits ADAM10 (a disintegrin and metalloproteinase domain-containing protein 10) protein. In HUVECs, the down-regulation of TIMP1 and up-regulation of MMP-3 results in aberrant endothelium-dependent vasodilation, EC (endothelial cell) death, and endothelial interruption in a FOXO3 (forkhead box O3)-mediated manner. In patients with CAD (coronary artery disease) and acute coronary syndrome (ACS), the urine levels of this protein are elevated. This protein interacts with Bcl-2 (B cell lymphoma) protein, and induces apoptosis in lung adenocarcinoma cells. The plasma levels of this protein are increased in patients with obesity and obesity-related disorders, such as steatosis, where it participates in pathogenesis of diet-induced hepatic steatosis and glucose intolerance. |
| Sequence【序列】 | CTCVPPHPQT AFCNSDLVIR AKFVGTPEVN QTTLYQRYEI KMTKMYKGFQ ALGDAADIRF VYTPAMESVC GYFHRSHNRS EEFLIAGKLQ DGLLHITTCS FVAPWNSLSL AQRRGFTKTY TVGCEECTVF PCLSIPCKLQ SGTHCLWTDQ LLQGSEKGFQ SRHLACLPRE PGLCTWQSLR SQIA |
| Physical form【外形】 | Lyophilized from 10 mM Sodium Phosphate, pH 7.5. |
| Reconstitution【重悬】 | Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/ml. Do not vortex. This solution can be stored at 2-8℃ for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20℃ to -80℃. |
产品性质
| biological source【生物来源】 | human |
| recombinant【重组】 | expressed in E. coli |
| Assay【测定】 | ≥95% (HPLC) ≥95% (SDS-PAGE) |
| form【形式】 | lyophilized |
| potency【效能】 | 0.5 μg/mL |
| mol wt【分子量】 | 20.6 kDa |
| packaging【包装】 | pkg of 10 μg |
| technique(s) | cell culture | mammalian: suitable |
| impurities【杂质】 | <0.1 EU/μg endotoxin, tested |
| color【颜色】 | white |
| UniProt accession no.【UniProt登记号】 | P01033 |
| shipped in【运输】 | wet ice |
| storage temp.【储存温度】 | −20℃ |
| Gene Information | human ... TIMP1(7076) |
安全信息
| Storage Class Code【储存分类代码】 | 13 - Non Combustible Solids |
| WGK | WGK 3 |
