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AKR1D1 human

产品编号:4103930
规格:recombinant, expressed in E. coli, ≥90% (SDS-PAGE)
包装规格:100 μG
产品类别:进口试剂
品牌:Sigma-Aldrich
优惠价:立即咨询
产品价格
产品编号包装单位单价(元)国内现货国外库存询价单
4103930100 μG5960
产品别名

AKR1D1 human

3o5bred

member D1

CBAS2

Aldo-keto reductase family 1

SRD5B1

产品性质
biological source【生物来源】
human
recombinant【重组】
expressed in E. coli
Assay【测定】
≥90% (SDS-PAGE)
form【形式】
liquid
mol wt【分子量】
39.5 kDa (346 aa, 1-326 aa + His Tag)
packaging【包装】
pkg of 100 μg
concentration【浓度】
0.5 mg/mL
UniProt accession no.【UniProt登记号】
P51857
shipped in【运输】
dry ice
storage temp.【储存温度】
−70℃
Gene Information
human ... AKR1D1(6718)
基本信息
General description【一般描述】
AKR1D1 (aldo-keto reductase 1D1) is the only steroid 5β-reductase in humans. This enzyme shows major expression in liver and resides in cytoplasm. It is a member of the unique subfamily, AKR1D, of the aldo-keto reductase (AKR) superfamily, that are NAD(P)(H)-dependent oxidoreductases. AKR1D1 contains the characteristic (α/β)8 triosephosphate isomerase barrel in its core region, and has a molecular weight of 37kDa. Its C-terminal contains the active site residing on the β strands, and the ligand binding site is formed by long flexible loops.
Recombinant human AKR1D1 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.
Biochem/physiol Actions【生化/生理作用】
AKR1D1 (aldo-keto reductase 1D1) catalyzes the reduction of Δ4-3-ketosteroids to 5β-dihydrosteroids in a stereospecific manner, and is responsible for inducing A/B cis-configuration of bile acids. It is a critical enzyme for steroid hormone metabolism (except estrogens) and bile acid biosynthesis. Mutations in this gene are linked with bile acid deficiency resulting in usually fatal neonatal hepatitis and cholestasis. Variants in this gene influence the expression of p450 enzymes, such as CYP3A4, CYP2C8, CYP2C9, CYP2C19, and CYP2B6, where up-regulation of AKR1D1 results in overexpression of these enzymes. Hence, this enzyme might indirectly be responsible for variation in drug metabolism or efficacy and adverse drug events in different individuals.
Physical form【外形】
0.5 mg/mL in 20 mM Tris-HCl buffer (pH 8.0) containing 1 mM DTT, 100 mM NaCl and 20% glycerol.
安全信息
Storage Class Code【储存分类代码】
13 - Non Combustible Solids
WGK
WGK 3
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