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AKT1 (E17K), Active
产品性质
| recombinant【重组】 | expressed in baculovirus infected Sf9 cells |
| Assay【测定】 | 70-95% (SDS-PAGE) |
| form【形式】 | frozen liquid |
| Specific Activity | 50-75 nmol/min·mg (radiometric) |
| mol wt【分子量】 | ~85 kDa |
| concentration【浓度】 | 0.1 μg/μL |
| accession no.【登记号】 | NM_005163 |
| shipped in【运输】 | dry ice |
| storage temp.【储存温度】 | −70℃ |
| Gene Information | human ... AKT1(207) |
基本信息
| Application【应用】 | Useful for kinase assay. |
| Biochem/physiol Actions【生化/生理作用】 | Recombinant full-length human AKT1 (E17K) was expressed by Baculovirus in Sf9 insect cells using an N-terminal GST tag. AKT1/PKBalpha is a serine/threonine kinase that belongs to the AKT family. AKT1 is activated in cells in response to diverse stimuli such as hormones, growth factors and extracellular matrix components and is involved in glucose metabolism, transcription, survival, cell proliferation, angiogenesis, and cell motility (1). AKT1 is frequently overexpressed and active in many types of human cancers including cancers of colon, breast, brain, pancreas and prostate as well as lymphomas and leukemias (2). |
| Reconstitution【重悬】 | Formulated in 50mM Tris-HCl, pH 7.5, 150mM NaCl, 10mM glutathione, 0.1mM EDTA, 0.25 mM DTT, 0.1 mM PMSF, with 25% glycerol. |
| Legal Information【法律信息】 | PRECISIO is a registered trademark of sigma-aldrich Co. LLC |
产品说明
| Storage and Stability【储存及稳定性】 | Store in aliquots at -70℃. Avoid freeze/thaw cycles. |
安全信息
| Storage Class Code【储存分类代码】 | 10 - Combustible liquids |
| WGK | WGK 1 |
