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Alkaline Phosphatase Assay Kit

产品编号:4103972
规格:Sufficient for 250 colorimetric tests
包装规格:1 kit
产品类别:进口试剂
品牌:Sigma-Aldrich
优惠价:立即咨询
产品价格
产品编号包装单位单价(元)国内现货国外库存询价单
41039721 kit4520
产品性质
Quality Level【质量水平】
100
application(s)
pharmaceutical
Detection Method
colorimetric
relevant disease(s)【相关疾病】
cancer; gastrointestinal diseases
storage temp.【储存温度】
−20℃
suitability【适用性】
The kit is suitable for the quantitative determination of alkaline phosphatase (ALP) activity in serum, plasma etc.
基本信息
General description【一般描述】
Alkaline phosphatase (ALP) catalyzes the hydrolysis of phosphate esters in an alkaline environment, resulting in the formation of an organic radical and inorganic phosphate. In mammals, this enzyme is found mainly in the liver and bones. Marked increase in serum ALP levels, a disease known as hyperalkalinephosphatasemia, has been associated with malignant biliary obstruction, primary biliary cirrhosis, primary sclerosing cholangitis, hepatic lymphoma and sarcoidosis.
Features and Benefits【特点和优势】
  • High sensitivity and wide linear range. The detection limit is 2 U/L, linear up to 800 U/L.
  • Simple "mix-and-measure" procedure allows reliable quantitation of ALP activity within 5 minutes.
  • Compatible with high-throughput handling systems.
Principle【原理】
The Alkaline Phosphatase Assay Kit is designed to measure ALP activity directly in biological samples without pretreatment. The improved method utilizes p-nitrophenyl phosphate that is hydrolyzed by ALP into a yellow colored product (maximal absorbance at A405 nm). The rate of the reaction isdirectly proportional to the enzyme activity.
安全信息
Pictograms【象形图】
GHS05,GHS07,GHS08,GHS09
Signal word【警示用语:】
Danger
Hazard Statements
H290 - H302 + H332 - H314 - H341 - H373 - H411
Precautionary Statements
P273 - P280 - P301 + P312 - P303 + P361 + P353 - P304 + P340 + P310 - P305 + P351 + P338
Hazard Classifications【危险分类】
Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Aquatic Chronic 2 - Eye Dam. 1 - Met. Corr. 1 - Muta. 2 - Skin Corr. 1B - STOT RE 2
Storage Class Code【储存分类代码】
8A - Combustible, corrosive hazardous materials
WGK
WGK 3
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