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Anti-Aggrecan Antibody, MMP Cleaved, NT FFGVG neoepitopes, clone AF-28

产品编号:4104693
规格:clone AF-28, Chemicon®, from mouse
包装规格:100 μG
产品类别:进口试剂
品牌:Sigma-Aldrich
优惠价:立即咨询
产品价格
产品编号包装单位单价(元)国内现货国外库存询价单
4104693100 μG6190
基本信息
eCl@ss
32160702
NACRES
NA.41
General description【一般描述】
Aggrecan is also known as aggregating chondroitin sulphate proteoglycan. Aggrecan is the major proteoglycan present in articular cartilage, composing up to 10% of its dry weight. It is responsible for endowing articular with its intrinsic properties of load bearing and compressive forces.
Specificity【特异性】
Mouse anti-aggrecan is a cleavage-site-specific monoclonal antibody for detecting metalloproteinase-derived aggrecan fragments. The antibody recognizes neo-epitopes on polypeptides with N-terminal FFGVG sequences. This sequence is found at the N-terminus of aggrecan fragments that have been digested with MMPs. By immunoblotting, AF-28 specifically detected G2 fragments derived from an aggrecan G1-G2 substrate digested with stromelysin, collagenase, gelatinase, and matrilysin, but failed to detect G2 fragments obtained from elastase, trypsin, or cathepsin B digests. Undigested G1-G2 was not detected. Competition experiments confirmed that peptides containing internal FFGVG sequences were not detected by the antibody. Clone AF-28 specifically recognizes a neo-epitope on polypeptides with N-terminal FFGVG Sequences. This sequence is found at the N-terminus of aggrecan fragments that have been digested with matrix metalloproteinases.
Immunogen【免疫原】
FFGVGGEEDC-KLH peptide
Epitope: N-terminus FFGVG neoepitopes
Application【应用】
Research Category
Cell Structure
Western blot

ELISA

Optimal working dilutions must be determined by the end user.
Research Sub Category
ECM Proteins

Inflammation & Autoimmune Mechanisms
Detect Aggrecan using this Anti-Aggrecan Antibody, MMP Cleaved, N-terminus FFGVG neoepitopes, clone AF-28 validated for use in ELISA & WB.
Physical form【外形】
Liquid in PBS pH 7.4 containing 0.09% sodium azide as a preservative.
Format: Purified
Protein A purified
Analysis Note【分析说明】
Control
Cartilage, neural tube, and brain tissue
Legal Information【法律信息】
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
产品性质
Quality Level【质量水平】
100
biological source【生物来源】
mouse
antibody form【抗体形式】
purified antibody
antibody product type
primary antibodies
clone【克隆】
AF-28, monoclonal
species reactivity
bovine, human, rat, mouse, pig
should not react with
guinea pig, horse
manufacturer/tradename
Chemicon®
technique(s)
ELISA: suitable
western blot: suitable
isotype【同位素/亚型】
IgG1
NCBI accession no.【NCBI登记号】
NM_001135.2
NM_013227.2
UniProt accession no.【UniProt登记号】
P16112
shipped in【运输】
wet ice
Gene Information
human ... ACAN(176)
产品说明
Storage and Stability【储存及稳定性】
Maintain for 1 year at 2–8°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Disclaimer【免责声明】
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
安全信息
Storage Class Code【储存分类代码】
10 - Combustible liquids
WGK
WGK 2
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