Anti-alpha Tubulin Antibody, tyrosinated

32160702

NA.41

Microtubules are involved in a wide variety of cellular activities ranging from mitosis and transport events to cell movement and the maintenance of cell shape. Tubulin itself is a globular protein which consists of two polypeptides (alpha and beta tubulin). Alpha and beta tubulin dimers are assembled to 13 protofilaments that form a microtubule of 22 nm diameter. Tyrosine ligase adds a C-terminal tyrosine to monomeric alpha tubulin. Assembled microtubules can be detyrosinated by a cytoskeleton associated carboxypeptidase. Detyrosinated alpha tubulin is referred to as Glu-tubulin. Another post-translational modification of detyrosinated alpha tubulin is C-terminal polyglutamylation which is characteristic for microtubules in neuronal cells and the mitotic spindle.

This antibody detects the tyrosinated form of alpha Tubulin.

KLH-conjugated linear peptide corresponding to human tyrosinated alpha Tubulin.

Western Blotting Analysis: A representative lot from an independent laboratory specifically detected tyrosinated alpha Tubulin and not nontyrosinated alpha Tubulin using purified porcine tubulin (Gundersen, G. G., et al. (1984). Cell. 38(3):779-89.).

ELISA Analysis: A representative lot from an independent laboratory specifically detected tyrosinated alpha Tubulin, in an indirect ELISA and in a competitive ELISA (Gundersen, G. G., et al. (1984). Cell. 38(3):779-89.).

Immunocytochemistry Analysis: A representative lot from an independent laboratory specifically detected tyrosinated alpha Tubulin and not nontyrosinated alpha Tubulin in TC-7 cells in interphase (Gundersen, G. G., et al. (1984). Cell. 38(3):779-89.).
Detect Tubulin using this rabbit polyclonal antibody, Anti-alpha Tubulin Antibody, tyrosinated validated for use in western blotting, ELISA & ICC.

Evaluated by Western Blotting in NIH/3T3 cell lysate, which were untreated or treated with Pancreatic Carboxypeptidase (PCA).

Western Blotting Analysis: A 1:2,000 dilution of this antibody detected alpha Tubulin, tyrosinated in 10 µg of untreated NIH/3T3 cell lysate and demonstrated a loss of signal in PCA treated NIH/3T3 cell lysate.

rabbit

100

serum

primary antibodies

polyclonal

mouse, monkey

rat (based on 100% sequence homology), human (based on 100% sequence homology)

ELISA: suitable
immunocytochemistry: suitable
western blot: suitable

NP_001257328

Q71U36

wet ice

human ... TUBA1A(7846)

~51 kDa observed. Uncharacterized band(s) may be observed in some cell lysates.

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

10 - Combustible liquids

WGK 1