Anti-APP A4 Antibody, a.a. 66-81 of APP {NT}, clone 22C11

32160702

NA.41

Deposits of amyloid protein in senile plaques near nerve processes are found in the brains of aged humand and cases of Alzheimer′s Disease. The principle component of this extracellular amyloid is beta A4, a 4 kDa peptide derived from a larger amyloid precursor protein (APP), which is widely expressed in the brain and body. The creation, transport and function of these proteins is currently an active area of research.

Reacts with pre-A4. The antibody recognizes amino acids 66-81 of the N-terminus on the pre-A4 molecule (Hilbich et al., 1993). 22C11 recognizes all three isoforms of APP, immature ~110kDa, sAPP ~120kDa, and mature ~130kDa (Hoffmann et al., 2000). The antibody is known to cross react with APLP2 (Slunt, 1994).

Epitope: a.a. 66-81 of APP {N-terminus}
Purified recombinant Alzheimer precursor A4 (pre A4695) fusion protein.

Immunofluorescence

Immunohistochemistry: 5-10 μg/mL on 4% paraformaldehyde/15% picric acid perfused and fixed rat spinal cord. 22C11 also works in paraffin embedded tissues but at lower dilutions (1:10-1:20).

Western blot: reducing conditions, 10μg/ml.

Optimal working dilutions and protocols must be determined by the end user.
Research Category
Neuroscience
Anti-APP A4 Antibody, a.a. 66-81 of APP {N-terminus}, clone 22C11 is an antibody against Alzheimer Precursor Protein A4 for use in IF, IH, IH(P) & WB with more than 85 product citations.
Research Sub Category
Neurodegenerative Diseases

Format: Purified
Protein A purified
Protein A Purified mouse immunoglobulin in 20 mM sodium phosphate, 250 mM NaCl, pH. 7.6, with 0.1% sodium azide as a preservative.

Control
Brain tissue, brain cell lysates

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

100

mouse

purified immunoglobulin

primary antibodies

22C11, monoclonal

human, fish, canine, monkey, pig, mouse, rat

Chemicon^®

immunofluorescence: suitable
immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable
western blot: suitable

IgG1

NM_000484.2
NM_201413.1
NM_201414.1

P05067

dry ice

human ... APP(351)

110/120/130 kDa

Maintain for 1 year at 2–8°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

12 - Non Combustible Liquids

WGK 2