Anti-Bacterial Peptidoglycan Antibody, clone 3F6B3

32160702

NA.41

Specific for the three-dimensional polymer complex structure of PG. The epitopes appear to consist of discontinuous glycan and/or amino acid residues, and have not been fully defined. In a competitive immunoassay format, several compounds were found to be ineffective as inhibitors: muramyldipeptide, N-acetylglucosamine (NAG), chitin (polymer of NAG) and acid-hydrolyzed chitin (NAG oligomers).

Insoluble PG obtained by TCA-heat and ethanol extraction of Streptococcus mutans BHT cells.

This Anti-Bacterial Peptidoglycan Antibody, clone 3F6B3 is validated for use in DB, ELISA, IC for the detection of Bacterial Peptidoglycan.
Research Category
Infectious Diseases
May be used in solid-phase or competitive ELISA, immunodot-blot and immunocytochemistry formats. Although PG is a bacterial surface component, pre-treatments, such as treatment with strong acid (25mM HCl), for Gram-positive bacteria, or with a detergent, such as SDS or Triton-X (1% in PBS), for Gram-negative bacteria, may be necessary to expose the epitopes.

Optimal working dilutions must be determined by end user.
Research Sub Category
Infectious Diseases - Viral

Ascites fluid, no preservatives. Liquid.

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

300

mouse

ascites fluid

primary antibodies

3F6B3, monoclonal

bacteria

Chemicon^®

ELISA: suitable
dot blot: suitable
immunocytochemistry: suitable

IgG1

wet ice

Maintain at -20°C in undiluted aliquots for up to 12 months. Avoid repeated freeze/thaw cycles.

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

10 - Combustible liquids

WGK 1